N,N'-bis-(azide-PEG3)-chlorocyclohexenyl Cy7 | CAS 2107273-84-5

N,N'-bis-(azide-PEG3)-chlorocyclohexenyl Cy7 is a Cy7-based fluorescent click handle designed for modular bioconjugation workflows, combining a near-infrared dye for deep-tissue-compatible imaging with two azide functional groups for strain-promoted or copper-free click coupling strategies. The chlorocyclohexenyl motif enables efficient cycloaddition-based assembly into labeled biomolecules and materials, while the PEG3 spacers help maintain conjugate accessibility and reduce steric constraints during probe construction and downstream imaging.

1. NIR Biomolecule Labeling

N,N'-bis-(azide-PEG3)-chlorocyclohexenyl Cy7 is used by chemical biology and fluorescence imaging groups to generate near-infrared labeled biomolecules for tracking, localization, and binding studies. Researchers typically incorporate the azide-bearing functionality into antibody, peptide, or protein conjugation pipelines where Cy7 readout is preferred for low-background fluorescence and compatibility with NIR detection systems. The PEG3 spacing supports efficient conjugation and improves labeling reproducibility when building multicomponent fluorescent reagents for microscopy, whole-well imaging, and instrument-based fluorescence readouts.

2. Multivalent Probe Construction

N,N'-bis-(azide-PEG3)-chlorocyclohexenyl Cy7 supports the assembly of multivalent fluorescent probes used in assay development and molecular imaging reagent design. By leveraging the dual azide functionality, teams can build higher-order conjugates that present multiple reactive sites for attaching targeting ligands, affinity moieties, or secondary reporters while maintaining Cy7 as the optical reporter. This approach is commonly applied when researchers need controlled stoichiometry for fluorescent signal generation in conjugate libraries, imaging probe optimization studies, or fluorescence-based characterization of engineered biomolecular systems.

3. Surface And Hydrogel Functionalization

N,N'-bis-(azide-PEG3)-chlorocyclohexenyl Cy7 is also applied in biomaterials research to introduce Cy7 fluorescence into surfaces, hydrogels, and polymer constructs through click-compatible functionalization strategies. Material scientists use the chlorocyclohexenyl and azide-bearing components to create fluorescently trackable coatings or bulk-incorporated labeling reagents for studying material-biomolecule interactions, diffusion/retention behavior, and spatial distribution within engineered matrices. The PEG3 linkers help preserve accessibility of reactive groups on polymer backbones, improving the practicality of incorporating the dye into complex material formats used for imaging and fluorescence quantification.