
LumiCell Reticulocyte Stain
| Catalog Number | A16-0224 |
| Category | Other Cell Fluorescent Probes |
| Molecular Formula | C19H17IN2S |
| Molecular Weight | 432.33 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
LumiCell Reticulocyte Stain is a ready-to-use solution of Thiazole Orange for determining a count of reticulocytes in human peripheral blood.
Product Specification
Application
Product Specification
| Excitation | 509 |
| Emission | 532 |
Application
LumiCell Reticulocyte Stain is a fluorescence-based staining reagent designed for labeling reticulocytes in blood-derived samples, enabling visualization and quantification of the immature red blood cell population. The formulation provides a convenient workflow for preparing stained cell suspensions compatible with common fluorescence readout formats, supporting routine hematology research, assay development, and method optimization in cell analysis laboratories.
1. Reticulocyte Flow Cytometry
LumiCell Reticulocyte Stain is used in flow cytometry workflows to quantify reticulocytes by generating a fluorescence signal that distinguishes immature erythroid cells from mature red blood cells. Researchers and core facilities commonly apply the stain during method development for reticulocyte counting, including optimization of staining time, buffer conditions, and instrument gating strategies for consistent population separation. The stained-cell format supports high-throughput sample processing for hematology research and workflow standardization in analytical laboratories.
2. Hematology Research Imaging
LumiCell Reticulocyte Stain is applied in fluorescence microscopy and imaging-based studies where reticulocyte enrichment and cellular heterogeneity need to be visualized in stained blood-derived preparations. Investigators use the reagent to support qualitative assessment of reticulocyte presence and distribution under experimental conditions, such as changes in erythropoiesis-related stimuli or sample handling variables. This imaging use case benefits from the stain's ability to provide a reticulocyte-associated fluorescence contrast that can be tracked across fields of view for experimental comparisons.
3. Reticulocyte Assay Development
LumiCell Reticulocyte Stain supports fluorescence assay development for researchers building reproducible reticulocyte quantification workflows. Teams in chemical biology, translational research platforms, and assay development groups use the reagent to evaluate staining robustness across sample types, prepare reference-stained controls, and refine analysis pipelines that rely on consistent fluorescence labeling of reticulocytes. In these workflows, the stain functions as a practical labeling component for generating assay-ready stained suspensions for downstream data acquisition and analysis.
4. Quality Control For Staining
LumiCell Reticulocyte Stain is frequently incorporated into laboratory quality control routines to verify staining consistency between runs, operators, and instrument sessions. Analytical teams use the reagent to prepare standardized stained controls that help monitor day-to-day variability in fluorescence readout and sample preparation steps. This application is especially relevant for laboratories performing longitudinal monitoring of reticulocyte populations in research studies where reproducible staining behavior is critical for reliable comparisons.
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