iFluor^® 700 Hydrazide

iFluor® 700 Hydrazide is a reactive fluorescent hydrazide designed for covalent labeling of carbonyl-containing biomolecules, enabling bright far-red fluorescence readouts in labeling workflows. The hydrazide functionality supports conjugation to aldehydes generated on glycans or oxidized carbohydrates, which is commonly leveraged to build fluorescent bioconjugates for imaging and analytical assays. Its far-red emission is frequently chosen to reduce spectral overlap with common visible fluorophores in multiplex fluorescence experiments.

1. Glycan Labeling For Imaging

iFluor® 700 Hydrazide is widely used to fluorescently tag glycans and glycoproteins for cellular and molecular imaging studies, where aldehyde-bearing carbohydrate motifs are introduced prior to conjugation. Researchers use the hydrazide to generate far-red fluorescent glycan conjugates that can be tracked in microscopy-based localization experiments, flow-based fluorescence readouts, and qualitative binding studies. This labeling strategy is particularly common in glyco-biology workflows that require robust covalent attachment of a fluorophore to carbohydrate-derived aldehydes while maintaining compatibility with downstream sample handling and imaging instrumentation.

2. Fluorescent Conjugate Synthesis

iFluor® 700 Hydrazide supports the preparation of fluorescent conjugates used as reagents in biochemical assays, imaging probes, and molecular tracing experiments. In practice, teams employ the hydrazide to couple iFluor® 700 to aldehyde-functional biomolecules, including carbohydrate-rich targets and oxidized biomolecular components, to create stable fluorescent reporters. Such conjugates are then used to monitor binding, uptake, distribution, or relative abundance in fluorescence microscopy, fluorescence-based plate assays, and other analytical formats where a far-red dye improves multiplexing flexibility.

3. Multiplex Far-Red Assays

iFluor® 700 Hydrazide is frequently selected when far-red spectral placement is required for multiplex fluorescence experiments that combine multiple fluorophores. In assay development workflows, the dye-labeled conjugates produced from aldehyde-reactive hydrazide chemistry help researchers integrate an additional fluorescence channel with reduced interference from many standard green and orange dyes. This makes iFluor® 700 hydrazide useful for designing multi-parameter imaging studies and fluorescence readouts in which careful spectral separation is needed to distinguish labeled targets within the same sample set.