Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite

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Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite

Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite | CAS 289712-99-8

Catalog Number A01-0019
Category Fluorescent Proteins & Nucleotides
Molecular Formula C79H89N6O17P
Molecular Weight 1425.57
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Product Introduction

Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite is a specialized nucleoside analog that incorporates a fluorescein moiety, known for its xanthene-based chromophore, into oligonucleotide synthesis. This compound features a 3'-CE phosphoramidite group, facilitating its integration into DNA or RNA strands during automated synthesis, thereby enabling the introduction of fluorescence for bioimaging and molecular tracking applications. Its structural design supports efficient energy transfer and fluorescence emission, making it suitable for use in fluorescence resonance energy transfer (FRET) studies and other fluorescence-based assays.

  • Chemical Information

  • Product Specification

Chemical Information

Related CAS 1194507-30-6 (6-Fluorescein dT phosphoramidite)
Synonyms Propanoic acid, 2,2-dimethyl-, 5-[[[6-[[3-[5-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-deoxy-β-D-erythro-pentofuranosyl]-3,4-dihydro-2,4-dioxo-1(2H)-pyrimidinyl]-1-oxo-2-propenyl]amino]hexyl]amino]carbonyl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester; Fluorescein-deoxy-pU Phosphoramidite; Fluorescein 5-methyl-2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite; 6-Fluorescein 5-methyl-2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite
Purity ≥95%
IUPAC Name [5-[6-[3-[5-[(2R,4S,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyoxolan-2-yl]-2,4-dioxopyrimidin-1-yl]prop-2-enoylamino]hexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate
SMILES CC(C)N(C(C)C)P(OCCC#N)OC1CC(OC1COC(C2=CC=CC=C2)(C3=CC=C(C=C3)OC)C4=CC=C(C=C4)OC)C5=CN(C(=O)NC5=O)C=CC(=O)NCCCCCCNC(=O)C6=CC7=C(C=C6)C8(C9=C(C=C(C=C9)OC(=O)C(C)(C)C)OC1=C8C=CC(=C1)OC(=O)C(C)(C)C)OC7=O
InChI InChI=1S/C79H89N6O17P/c1-49(2)85(50(3)4)103(96-42-20-38-80)102-67-46-64(99-68(67)48-95-78(52-21-16-15-17-22-52,53-24-28-55(93-11)29-25-53)54-26-30-56(94-12)31-27-54)60-47-84(75(92)83-71(60)88)41-37-69(86)81-39-18-13-14-19-40-82-70(87)51-23-34-61-59(43-51)72(89)101-79(61)62-35-32-57(97-73(90)76(5,6)7)44-65(62)100-66-45-58(33-36-63(66)79)98-74(91)77(8,9)10/h15-17,21-37,41,43-45,47,49-50,64,67-68H,13-14,18-20,39-40,42,46,48H2,1-12H3,(H,81,86)(H,82,87)(H,83,88,92)/t64-,67+,68-,103?/m1/s1
InChIKey WHVKLEUMJJAXRZ-PCLSEREPSA-N
Appearance Off-white powder

Product Specification

Storage Store at 2-8 °C

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