
Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite | CAS 289712-99-8
| Catalog Number | A01-0019 |
| Category | Fluorescent Proteins & Nucleotides |
| Molecular Formula | C79H89N6O17P |
| Molecular Weight | 1425.57 |
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Product Introduction
Fluorescein 2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite is a specialized nucleoside analog that incorporates a fluorescein moiety, known for its xanthene-based chromophore, into oligonucleotide synthesis. This compound features a 3'-CE phosphoramidite group, facilitating its integration into DNA or RNA strands during automated synthesis, thereby enabling the introduction of fluorescence for bioimaging and molecular tracking applications. Its structural design supports efficient energy transfer and fluorescence emission, making it suitable for use in fluorescence resonance energy transfer (FRET) studies and other fluorescence-based assays.
Chemical Information
Product Specification
Chemical Information
| Related CAS | 1194507-30-6 (6-Fluorescein dT phosphoramidite) |
| Synonyms | Propanoic acid, 2,2-dimethyl-, 5-[[[6-[[3-[5-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-deoxy-β-D-erythro-pentofuranosyl]-3,4-dihydro-2,4-dioxo-1(2H)-pyrimidinyl]-1-oxo-2-propenyl]amino]hexyl]amino]carbonyl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester; Fluorescein-deoxy-pU Phosphoramidite; Fluorescein 5-methyl-2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite; 6-Fluorescein 5-methyl-2'-deoxy-5'-O-DMT-pseudouridine 3'-CE phosphoramidite |
| Purity | ≥95% |
| IUPAC Name | [5-[6-[3-[5-[(2R,4S,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyoxolan-2-yl]-2,4-dioxopyrimidin-1-yl]prop-2-enoylamino]hexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate |
| SMILES | CC(C)N(C(C)C)P(OCCC#N)OC1CC(OC1COC(C2=CC=CC=C2)(C3=CC=C(C=C3)OC)C4=CC=C(C=C4)OC)C5=CN(C(=O)NC5=O)C=CC(=O)NCCCCCCNC(=O)C6=CC7=C(C=C6)C8(C9=C(C=C(C=C9)OC(=O)C(C)(C)C)OC1=C8C=CC(=C1)OC(=O)C(C)(C)C)OC7=O |
| InChI | InChI=1S/C79H89N6O17P/c1-49(2)85(50(3)4)103(96-42-20-38-80)102-67-46-64(99-68(67)48-95-78(52-21-16-15-17-22-52,53-24-28-55(93-11)29-25-53)54-26-30-56(94-12)31-27-54)60-47-84(75(92)83-71(60)88)41-37-69(86)81-39-18-13-14-19-40-82-70(87)51-23-34-61-59(43-51)72(89)101-79(61)62-35-32-57(97-73(90)76(5,6)7)44-65(62)100-66-45-58(33-36-63(66)79)98-74(91)77(8,9)10/h15-17,21-37,41,43-45,47,49-50,64,67-68H,13-14,18-20,39-40,42,46,48H2,1-12H3,(H,81,86)(H,82,87)(H,83,88,92)/t64-,67+,68-,103?/m1/s1 |
| InChIKey | WHVKLEUMJJAXRZ-PCLSEREPSA-N |
| Appearance | Off-white powder |
Product Specification
| Storage | Store at 2-8 °C |
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