N,N'-[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar',ar'-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine] | 154071-48-4

N,N’-[(3’,6’-Dihydroxy-3-oxospiro[isobenzofuran-1(3H)9’-[9H]xanthene]-ar’ar’-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine], a complex chemical compound with a myriad of applications, particularly shines as a fluorescent dye. Here are four key applications of this compound presented with high perplexity and burstiness:

Fluorescence Microscopy: Operating at the forefront of scientific exploration, this compound serves as a staple fluorescent dye in a multitude of microscopy techniques. Its unique properties unleash vibrant visible light upon specific wavelength excitation, enabling the visualization of intricate cellular structures and processes. By labeling organelles, proteins, and biomolecules, researchers delve into the dynamic realm of cellular dynamics and interactions, capturing real-time insights with unparalleled depth.

Flow Cytometry: In the realm of flow cytometry, this fluorescent dye emerges as a powerful tool for labeling and distinguishing diverse cell populations based on their distinct fluorescent characteristics. Cells adorned with this dye undergo rigorous analysis for surface markers, intracellular molecules, and other defining traits. This application plays a pivotal role in immunophenotyping, cell sorting, and a myriad of diagnostic procedures in both clinical and research domains, offering a gateway to in-depth cellular exploration.

Molecular Probes: Embracing the intricate dance of biochemistry, this compound takes center stage as a molecular probe in biochemical assays adept at detecting the presence of specific ions or molecules. By coupling the dye with antibodies or other affinity reagents, researchers unlock the ability to quantify target substances within complex mixtures. This versatility finds particular significance in enzyme-linked immunosorbent assays (ELISAs) and other bioanalytical techniques, offering a lens into the intricate molecular landscape.

Live Cell Imaging: A cornerstone in the realm of live cell imaging, N,N’-[(3’,6’-Dihydroxy-3-oxospiro[isobenzofuran-1(3H)9’-[9H]xanthene]-ar’ar’-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine] stands poised to illuminate dynamic biological processes within living cells. This compound facilitates the exploration of cellular responses to stimuli, protein trafficking dynamics, and intracellular signaling pathways. Through this application, researchers gain a profound understanding of complex biological mechanisms and the effects of drugs in real-time, unravelling the intricacies of life’s molecular choreography.