FAM-11-UTP, 6-isomer

FAM-11-UTP, 6-isomer is a fluorescein (FAM)-labeled uridine triphosphate analog designed for fluorescent nucleic acid labeling workflows. As a UTP derivative, it is incorporated by polymerases into RNA during in vitro transcription, enabling direct fluorescent readout without post-labeling steps. The fluorescein reporter supports visualization and quantitative fluorescence measurements in nucleic acid assays and molecular imaging experiments.

1. In Vitro RNA Transcription Labeling

FAM-11-UTP, 6-isomer is used by molecular biology and RNA technology groups to generate fluorescently labeled RNA transcripts for downstream imaging and fluorescence-based analyses. Researchers incorporate the analog during in vitro transcription to produce FAM-tagged RNA that can be tracked in hybridization experiments, binding studies, and fluorescence microscopy workflows. This approach is particularly valuable when a consistent, covalently attached fluorophore is needed across RNA populations for reproducible fluorescence readouts.

2. Fluorescent RNA Hybridization Probes

FAM-11-UTP, 6-isomer supports the construction of fluorescent RNA probes used in nucleic acid hybridization assays. By generating FAM-labeled RNA targets or probe RNAs, labs can monitor hybridization events through fluorescence detection rather than relying on radioactive labeling. Common use cases include mapping RNA-RNA interactions, validating complementary sequence recognition, and developing fluorescence readouts for nucleic acid binding studies in plate-based or imaging formats.

3. Fluorescence-Based RNA Quantification

FAM-11-UTP, 6-isomer is frequently applied in fluorescence quantification workflows where labeled RNA provides a direct signal proportional to RNA abundance. Researchers use FAM-labeled transcripts as standards or assay components in fluorescence measurements to compare transcription outputs, assess labeling consistency, or monitor RNA processing steps in vitro. This reagent is also used to support assay development for nucleic acid workflows that benefit from real-time or endpoint fluorescence readout using standard fluorescence instrumentation.

4. RNA Localization Microscopy Studies

FAM-11-UTP, 6-isomer enables cellular and molecular imaging studies that track RNA localization using fluorescein-tagged transcripts. By producing FAM-labeled RNA via in vitro transcription, researchers can visualize RNA distribution in imaging experiments where covalent fluorophore incorporation improves consistency compared with noncovalent dyes. The fluorescein reporter is leveraged to support fluorescence microscopy readouts for RNA trafficking, uptake, and interaction studies in model experimental systems.