endo-BCN-PEG2-alcohol | CAS 1807501-85-4

endo-BCN-PEG2-alcohol is a cyclooctyne-based click chemistry reagent designed for strain-promoted azide–alkyne cycloaddition (SPAAC) with azide-functional biomolecules and materials. The endo-BCN scaffold provides rapid, catalyst-free reactivity under aqueous-compatible conditions, while the PEG2-alcohol handle improves solubility and supports conjugation workflows where a hydroxyl-terminated linker is advantageous. This reagent is commonly used in chemical biology and biomaterials research to install or label azide-bearing targets with minimal perturbation, enabling modular probe and material assembly.

1. Live-Cell Imaging Labeling

endo-BCN-PEG2-alcohol is widely used to visualize azide-tagged biomolecules in cellular and imaging workflows where copper-free click chemistry is preferred to avoid metal-associated artifacts. Researchers incorporate azide groups into proteins, glycans, or nucleic-acid analogs and then use endo-BCN-PEG2-alcohol to generate fluorescent or affinity-tagged conjugates suitable for microscopy and imaging readouts. The PEG2-alcohol component supports dispersion in aqueous media and helps maintain probe performance during staining and wash steps, making the reagent a practical choice for iterative labeling experiments and multi-step sample preparation.

2. Biomolecule Conjugation Workflows

endo-BCN-PEG2-alcohol supports modular bioconjugation of azide-bearing biomolecules used in proteomics, chemical proteomics, and affinity reagent development. The reagent’s endo-BCN reactivity enables efficient attachment to azide-functional ligands, peptides, or scaffold proteins, allowing researchers to generate conjugates for downstream assays such as pull-down experiments, binding studies, and analytical characterization. The PEG2-alcohol handle is particularly useful when a hydrophilic linker is needed to improve handling, reduce nonspecific interactions, or provide a convenient functional motif for further derivatization in complex labeling pipelines.

3. Surface and Hydrogel Functionalization

endo-BCN-PEG2-alcohol is employed to functionalize polymer surfaces, coatings, and hydrogels that present azide groups, enabling the creation of SPAAC-linked material architectures. In biomaterials development, azide-functional substrates are reacted with endo-BCN-PEG2-alcohol to immobilize bioactive motifs, imaging handles, or affinity ligands without requiring copper catalysts, which can be incompatible with sensitive materials or processing conditions. The PEG2-alcohol segment contributes to improved wetting and uniform conjugation behavior across aqueous fabrication steps, supporting reproducible material modification for research-grade platforms.

4. Diagnostic Reagent and Assay Design

endo-BCN-PEG2-alcohol is used in the construction of diagnostic and assay reagents where azide-tagged capture molecules, reporters, or nanoparticles are assembled via catalyst-free click chemistry. Assay developers rely on SPAAC to couple endo-BCN-bearing reagents to azide-functional components during probe preparation, enabling consistent conjugation of detection labels, affinity reagents, or signal-generating constructs. The PEG2-alcohol functionality helps maintain reagent solubility during buffer-based workflows and supports scalable coupling strategies for multiplexed assay formats and standardized reagent kits used in research environments.