Cyanine7.5 amine | CAS 2104005-15-2

Cyanine7.5 amine is a reactive cyanine dye building block designed for fluorescent labeling workflows. As an amine-functionalized fluorophore, it is commonly used to prepare dye conjugates for fluorescence imaging and fluorescence-based assays, where excitation in the far-red region and bright emission enable low-background visualization in labeled biological samples. The amine handle supports downstream conjugation to biomolecules and polymers using standard bioconjugation strategies.

1. Protein And Antibody Labeling

Cyanine7.5 amine is frequently used by chemical biology and bioconjugation labs to generate far-red fluorescent antibody and protein conjugates for microscopy and plate-based fluorescence readouts. Researchers typically incorporate the amine functionality into labeling schemes that produce stable dye-biomolecule conjugates for tracking binding interactions, monitoring biomolecule localization, and building fluorescent reagents for assay development. Far-red emission is especially useful when multiplexing with green/orange channels or when minimizing interference from autofluorescence in complex biological matrices.

2. Fluorescence Microscopy Tracing

Cyanine7.5 amine supports cellular and tissue imaging workflows where far-red fluorescence helps improve contrast and visual separation from endogenous signals. Imaging teams use dye conjugates derived from the amine handle to label biomolecule targets, study transport and distribution in fixed samples, and generate fluorescent tracers for localization experiments. The dye's cyanine class photophysics are leveraged in fluorescence microscopy to visualize labeled structures over extended imaging sessions during standard lab acquisition protocols.

3. Oligonucleotide And Probe Conjugation

Cyanine7.5 amine is used in molecular imaging reagent development to create fluorescently labeled nucleic acid probes and oligonucleotide conjugates for fluorescence readouts. Molecular biology groups incorporate the amine functionality into probe construction to enable visualization of hybridization products, tracking of labeled strands, and fluorescence-based nucleic acid analysis workflows. The far-red spectral window is often chosen to reduce spectral overlap with common fluorophores used in multi-color nucleic acid experiments.

4. Polymer to Biomaterial Labeling

Cyanine7.5 amine is also applied in biomaterials science to introduce far-red fluorescent tags into polymers, hydrogels, and surface-modified materials for imaging-guided materials characterization. Materials researchers use dye conjugation to enable visualization of coatings, track material distribution in experimental systems, and support fluorescence-based studies of biomaterial interfaces. The amine functional group provides a convenient reactive handle for preparing fluorescent material derivatives used in microscopy and fluorescence assay development.