Coumarin 515 | CAS 41044-12-6

Name: Coumarin 515
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	A17-0073
Category	Laser Dyes
Molecular Formula	C21H21N3O2
Molecular Weight	347.41

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Product Introduction

High-yield fluorescent dye with intense emission in the green region. Excellent for fluorescence imaging and optical materials.

Chemical Information
Application

Synonyms	7-(diethylamino)-3-(1-methyl-1H-benzimidazol-2-yl)-2H-1-benzopyran-2-one; Coumarin 30; 3-(2-N-Methylbenzimidazolyl)-7-N,N-diethylaminocoumarin
Purity	>98.0%
Canonical SMILES	CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)O2)C3=NC4=CC=CC=C4N3C
InChI	InChI=1S/C21H21N3O2/c1-4-24(5-2)15-11-10-14-12-16(21(25)26-19(14)13-15)20-22-17-8-6-7-9-18(17)23(20)3/h6-13H,4-5H2,1-3H3
InChIKey	KZFUMWVJJNDGAU-UHFFFAOYSA-N
Appearance	Yellow Solid
Melting Point	223 °C

Coumarin 515, a versatile fluorescent dye widely utilized in scientific research, exhibits a myriad of applications characterized by high perplexity and burstiness:

Fluorescence Microscopy: Serving as a prevalent fluorescent label in microscopy, Coumarin 515 stands out for its vivid fluorescence and exceptional photostability, rendering it ideal for extensive imaging studies. Researchers employ it to stain organelles, track cellular dynamics, and monitor biomolecular movements within cells, unveiling a world of intricate cellular structures and functions.

Flow Cytometry: In the realm of flow cytometry, Coumarin 515 acts as a distinctive fluorescent tag, enabling the differentiation and analysis of diverse cell populations. Its unique emission wavelength facilitates multiplexing with other fluorophores, facilitating comprehensive phenotypic assessments. This application aids in identifying cell types, measuring protein expression levels, and evaluating cell viability across various biological samples, enhancing analytical capabilities.

Fluorescence Resonance Energy Transfer (FRET): Pioneering FRET-based methodologies, Coumarin 515 plays a pivotal role in studying biomolecular interactions. Coupled with suitable donor or acceptor dyes, researchers leverage Coumarin 515 to quantify molecular associations and real-time conformational changes. This technique proves invaluable in unraveling protein-protein interactions, enzyme activities, and dynamic biological processes, offering insights into complex molecular behaviors.

Environmental Sensing: At the forefront of environmental sensing, Coumarin 515 emerges as a versatile probe for monitoring environmental factors. Its fluorescence properties undergo alterations in response to specific environmental variables like pH, temperature, and the presence of distinct ions or molecules. This adaptability enables the development of sensitive assays and sensors for detecting pollutants, toxins, and other environmental elements, enriching our capacity for environmental monitoring and safety.