Coumarin 480 | 41267-76-9

Coumarin 480, a fluorescent compound renowned for its photophysical properties, finds widespread utilization across diverse bioscience applications. Here are four key applications of Coumarin 480:

Fluorescent Labeling: Within biological labeling endeavors, Coumarin 480 emerges as a beacon of fluorescence deployed to detect and quantify biomolecules in a myriad of assays. Researchers employ this compound to illuminate cellular components like proteins, nucleic acids, and membranes in fluorescence microscopy, enabling high-contrast imaging and precise spatial localization within intricate biological specimens.

Fluorescence Resonance Energy Transfer (FRET) Studies: Serving as a potent FRET donor, Coumarin 480 boasts spectral properties conducive to probing energy transfers between donor and acceptor molecules. This technique serves as a window into molecular interactions and dynamic conformational changes unfolding in real-time. Coupled with suitable acceptors, scientists harness the power of Coumarin 480 to monitor intricate biological processes with unparalleled sensitivity.

Environmental Sensing: In the realm of environmental monitoring, Coumarin 480 plays a pivotal role in sensors engineered to detect shifts in pH or ion concentrations, owing to its fluorescence responsiveness under diverse conditions. Whether integrated into polymer matrices or nanoparticles, this compound powers the creation of responsive probes, facilitating real-time surveillance for both environmental and industrial contexts.

High-Throughput Screening: At the forefront of pharmaceutical and biotechnological endeavors, Coumarin 480 is a cornerstone in high-throughput screening assays, fueling drug discovery and development efforts. Its robust fluorescence emission enables swift and quantitative evaluation of compound libraries against biological targets, expediting the identification of potential therapeutic candidates and catalyzing the drug development pathway.