VIC phosphoramidite, 6-isomer | CAS 1414265-81-8

VIC phosphoramidite, 6-isomer is a fluorescent phosphoramidite building block used to introduce the VIC dye into oligonucleotides during solid-phase DNA synthesis. As a dye-labeling reagent for nucleic acid workflows, it is commonly selected for fluorescence-based genotyping and fragment analysis formats where VIC provides a distinct signal channel compatible with standard capillary and microfluidic detection systems. The phosphoramidite form enables efficient incorporation at defined positions, supporting assay design for probe labeling, primer labeling, and internal reference strategies in nucleic acid analysis.

1. Genotyping And Fragment Analysis

VIC phosphoramidite, 6-isomer is widely used in oligonucleotide labeling for genotyping assays and DNA fragment sizing workflows, including SNP discrimination and allele calling formats. Researchers incorporate the VIC label into primers or probes so that amplification or hybridization products generate a fluorescence readout that can be separated by instrument optics and analyzed as discrete peaks. This labeling approach is especially common in capillary electrophoresis and microfluidic fragment analysis workflows where VIC dye emission is leveraged as a dedicated detection channel for multiplexed nucleic acid panels.

2. qPCR And Real-Time Amplification Probes

VIC phosphoramidite, 6-isomer is used to construct fluorescence-based real-time PCR reagents by installing the VIC dye at specific oligonucleotide positions. In probe-based qPCR designs, the VIC-labeled oligonucleotide serves as the fluorescence reporter component whose signal changes are tracked during thermal cycling, enabling quantitative monitoring of target amplification in assay development and method optimization. Oligonucleotide synthesis incorporating this phosphoramidite supports consistent probe architecture for research-grade assay panels and screening workflows that require defined reporter placement.

3. Multiplex Nucleic Acid Assay Design

VIC phosphoramidite, 6-isomer supports multiplex nucleic acid assay development by enabling the creation of VIC-labeled oligonucleotides that occupy a distinct fluorescence channel alongside other reporter dyes. Assay developers use VIC-labeled primers/probes to build multi-target workflows where multiple analytes are resolved in a single run based on dye-specific detection. The phosphoramidite functionality is particularly valuable when a defined dye position is needed to maintain probe performance and to align with instrument filter sets used for multiplex fluorescence readout.

4. Fluorescent Oligonucleotide Standards

VIC phosphoramidite, 6-isomer is also employed to prepare fluorescently labeled oligonucleotide standards for assay calibration and method validation in nucleic acid analysis. By incorporating VIC at predetermined sites, researchers generate reference materials that help verify labeling consistency, instrument channel assignment, and run-to-run comparability in fluorescence detection workflows. These standards are frequently used during assay setup for fragment analysis and fluorescence-based nucleic acid quantification experiments.