Oxazine 750 Perchlorate | CAS 85256-40-2

Oxazine 750 Perchlorate is a bright oxazine-family far-red/near-infrared-emitting fluorescent dye commonly used as a labeling and imaging reagent. Its strong absorption and emission make it useful for fluorescence microscopy and optical detection workflows, while its dye scaffold supports convenient conjugate preparation for tracking biomolecules and materials. In practice, Oxazine 750 Perchlorate is handled as a fluorophore building block for developing fluorescent stains, conjugates, and assay reagents where far-red readout helps reduce background from shorter-wavelength autofluorescence.

1. Fluorescence Microscopy Labeling

Oxazine 750 Perchlorate is frequently used by microscopy groups to generate far-red fluorescent labeling reagents for cellular and biomolecular imaging. Researchers incorporate the dye into conjugates for staining workflows that benefit from reduced spectral interference compared with green/yellow fluorophores, supporting clearer visualization of labeled structures in fixed-cell experiments and fluorescence imaging studies. In fluorescence microscopy pipelines, the dye is also used as a convenient fluorophore for preparing custom imaging probes for co-localization experiments with other spectrally separated reporters.

2. Flow Cytometry Fluorescent Tagging

Oxazine 750 Perchlorate is used in flow cytometry development as a far-red fluorescent tag for labeling biomolecules and preparing stained samples for instrument-based quantification. Typical workflows include generating fluorescent conjugates that can be used as detection reagents in cell staining panels where spectral spacing helps minimize spillover from other fluorophores. Flow cytometry users value Oxazine 750 Perchlorate when constructing multi-color staining strategies that rely on robust far-red emission for gating and population analysis.

3. Fluorescent Conjugate Standards

Oxazine 750 Perchlorate is widely applied in fluorescence assay development and instrumentation support as a reference fluorophore for preparing working standards and calibration materials. In laboratories that develop fluorescence-based readouts, the dye is used to verify excitation/emission performance, normalize signal across experiments, and support method development for far-red fluorescence detection platforms. Researchers also use Oxazine 750 Perchlorate-derived conjugates or dye-containing controls to benchmark assay reagents and imaging reagents during optimization of labeling density and measurement conditions.

4. Biomaterial Surface Staining

Oxazine 750 Perchlorate is employed in materials and biomaterials research to visualize and quantify fluorescently labeled surfaces, coatings, and immobilized biomolecule layers. Investigators incorporate the dye into surface-associated labeling strategies to confirm coating uniformity, track adsorption or immobilization of functional components, and support fluorescence-based characterization of modified materials. This use is common in studies where far-red fluorescence provides cleaner contrast for imaging thin films, polymer surfaces, or labeled interfaces under optical microscopy or plate-based fluorescence readout.