Oxazine 170 perchlorate | CAS 62669-60-7

Catalog Number	A17-0105
Category	Laser Dyes
Molecular Formula	C21H22N3O∙ClO4
Molecular Weight	431.87

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Product Introduction

Oxazine 170 perchlorate is a pivotal biomedical dye extensively utilized in the domains of fluorescence microscopy and flow cytometry. It exhibits immense potential in the labeling of nucleic acids and proteins. Notably, this dye facilitates the visual apprehension of intricate cellular processes, notably shedding light on the mechanisms fueling cellular uptake and the dynamic behavior of cellular constituents.

Chemical Information
Application

Synonyms	5-(ethylimino)-10-Methyl-5H-benzo[a]phenoxazin-9-ethylamine mono-perchlorate; Oxazine 720 Perchlorate
Canonical SMILES	CCNC1=CC2=C(C3=CC=CC=C31)N=C4C=C(C(=[NH+]CC)C=C4O2)C.[O-]Cl(=O)(=O)=O
InChI	InChI=1S/C21H21N3O.ClHO4/c1-4-22-16-11-19-18(10-13(16)3)24-21-15-9-7-6-8-14(15)17(23-5-2)12-20(21)25-19;2-1(3,4)5/h6-12,23H,4-5H2,1-3H3;(H,2,3,4,5)
InChIKey	CBXAZZAYBZVPEZ-UHFFFAOYSA-N

Oxazine 170 perchlorate is a synthetic dye with significant utility in biological, medical, and industrial applications. Here are some key applications of Oxazine 170 perchlorate:

Fluorescent Microscopy: Oxazine 170 perchlorate is widely used as a fluorescent dye in microscopy to stain and visualize biological structures. Its strong fluorescence emission allows for the detailed imaging of cellular components, such as membranes and organelles. This aids researchers in studying cellular morphology and dynamics with high specificity and clarity.

Flow Cytometry: In flow cytometry, Oxazine 170 perchlorate is utilized as a fluorescent marker to label cells and analyze their properties. It helps in the identification and quantification of specific cell populations based on fluorescence signals. This application is essential for various clinical and research purposes, such as immunophenotyping and cell sorting.

Nucleic Acid Staining: Oxazine 170 perchlorate can be used to stain nucleic acids, making it valuable in molecular biology experiments. By binding to DNA or RNA, it enables the visualization of nucleic acids in techniques like gel electrophoresis and fluorescence in situ hybridization (FISH). This facilitates the study of genetic material and gene expression.

Laser-Induced Fluorescence: In analytical chemistry, Oxazine 170 perchlorate is applied in laser-induced fluorescence (LIF) techniques for the detection of various compounds. Its high quantum yield and photostability make it suitable for sensitive and accurate measurements. LIF using Oxazine 170 perchlorate can be employed in environmental monitoring, clinical diagnostics, and biochemical assays.