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NADA-green | CAS 2253733-11-6
| Catalog Number | A21-0007 |
| Category | Fluorescent Probes for Imaging Bacteria |
| Molecular Formula | C9H9N5O5.HCl |
| Molecular Weight | 303.66 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
NADA-green is a fluorescent dopamine analog for imaging dopaminergic neuron activity. It allows real-time visualization of neurotransmitter release.
Chemical Information
Product Specification
Application
Computed Properties
| Synonyms | 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride |
| Purity | ≥98% by HPLC |
| IUPAC Name | (2R)-2-amino-3-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]propanoic acid;hydrochloride |
| Canonical SMILES | C1=C(C2=NON=C2C(=C1)[N+](=O)[O-])NCC(C(=O)O)N.Cl |
| InChI | InChI=1S/C9H9N5O5.ClH/c10-4(9(15)16)3-11-5-1-2-6(14(17)18)8-7(5)12-19-13-8;/h1-2,4,11H,3,10H2,(H,15,16);1H/t4-;/m1./s1 |
| InChIKey | NKFVSANWNYYLLB-PGMHMLKASA-N |
| Solubility | DMSO |
| Excitation | 450 nm |
| Emission | 555 nm |
| Storage | Store at -20°C |
NADA-green, a fluorescent dye prevalent in biological and biochemical research, boasts versatile applications. Here are four key uses of NADA-green:
Immunofluorescence: Through the utilization of NADA-green, researchers can tag antibodies, enabling the visualization of specific proteins’ presence and distribution within cells and tissues using a fluorescence microscope. This technique is fundamental for scrutinizing protein localization and interactions, offering remarkable specificity and sensitivity in detecting target molecules amidst intricate biological samples.
Live Cell Imaging: Renowned for its low toxicity and robust fluorescence properties, NADA-green shines in live cell imaging endeavors. Scientists leverage this dye to track dynamic biological processes like cellular signaling, migration, and division in real-time, facilitating the exploration of cellular functions within their natural contexts and providing invaluable insights into cellular behavior and physiology.
Flow Cytometry: Embraced in flow cytometry settings, NADA-green serves to label and identify cells or particles based on specific attributes. This enables the simultaneous analysis of diverse parameters encompassing cell surface markers and intracellular proteins across a vast cell population. Such applications are indispensable for tasks ranging from immunophenotyping to cell sorting, proving pivotal in diagnostic and research landscapes.
Fluorescence-Based Assays: Within various fluorescence-based assays, NADA-green emerges as a stalwart tool for measuring biochemical activities such as enzyme activities, ligand-receptor interactions, and nucleic acid hybridization. Its vibrant fluorescence signature furnishes a dependable readout for quantifying these activities in high-throughput screening and analytical assays, thereby catalyzing drug discovery efforts and unraveling intricate molecular mechanisms.
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 4 |
| Exact Mass | 303.0370461 g/mol |
| Monoisotopic Mass | 303.0370461 g/mol |
| Topological Polar Surface Area | 160Ų |
| Heavy Atom Count | 20 |
| Formal Charge | 0 |
| Complexity | 361 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 1 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 2 |
| Compound Is Canonicalized | Yes |
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