MitoHunt Red CM-H2Xros | CAS 167095-08-1

MitoHunt Red CM-H2Xros is a mitochondria-targeted fluorescent staining reagent designed for monitoring cellular mitochondrial oxidative activity through fluorescence turn-on behavior. The CM-H2Xros dye format incorporates an electrophile-reactive, cell-compatible scaffold that forms a fluorescent product preferentially in mitochondria, enabling visualization of mitochondrial redox state changes by fluorescence microscopy and imaging workflows. Its red emission supports multiplex-friendly experimental designs when paired with compatible far-red or green channels.

1. Mitochondrial Redox Imaging

MitoHunt Red CM-H2Xros is used in fluorescence microscopy and plate-based imaging to visualize mitochondrial oxidative activity and track redox-dependent changes in living cells under controlled experimental conditions. Researchers commonly apply it to compare mitochondrial responses across treatments, time courses, and cell models, leveraging its mitochondria-enriched signal to interpret spatial patterns of oxidative stress within the mitochondrial network. The red fluorescence output is particularly convenient for multicolor imaging panels where green and far-red channels are already allocated to other probes or labels.

2. Live-Cell Fluorescence Assays

MitoHunt Red CM-H2Xros supports quantitative fluorescence readouts in live-cell assay development, including screening-style experiments in which mitochondrial oxidative activity is used as a functional reporter. In practice, investigators use the reagent to generate time-resolved fluorescence intensity metrics that reflect mitochondrial redox shifts, often normalizing to cell number or parallel viability indicators to support robust assay workflows. The dye's mitochondria-focused staining behavior helps reduce ambiguity from cytosolic background, making it a practical choice for establishing reproducible imaging assays.

3. Fluorescence Microscopy Co-Localization

MitoHunt Red CM-H2Xros is frequently paired with mitochondrial structural markers, membrane potential dyes, or cytosolic reporters to study how mitochondrial oxidative activity relates to organelle morphology and subcellular organization. Imaging teams use it for co-localization experiments that map redox-responsive fluorescence onto specific mitochondrial features, supporting mechanistic studies of mitochondrial dynamics and stress responses. Its red emission channel is useful for designing microscopy experiments that require spectral separation between mitochondrial activity readouts and other fluorescent labels.

4. Flow Cytometry Mitochondrial Readout

MitoHunt Red CM-H2Xros can be incorporated into flow cytometry workflows to quantify mitochondria-associated fluorescence across large cell populations. Researchers use it to assess shifts in mitochondrial oxidative activity as a population-level signal, enabling comparisons between experimental groups without relying solely on single-cell imaging. This application is commonly used in cell biology and chemical biology studies where mitochondrial functional readouts are required alongside other fluorescence parameters for gating, phenotyping, and multivariate analysis.