LDS 765 | CAS 106025-69-8

LDS 765 is a cyanine-derived fluorescent dye used as a far-red/near-infrared molecular imaging label for fluorescence-based detection workflows. Its strong absorption and emission in the red spectral region make it practical for optical tracing and imaging experiments where reduced background from biological autofluorescence is advantageous. The dye is commonly handled as a reactive or conjugatable fluorophore building block for preparing fluorescent conjugates, probes, and labeling reagents used in microscopy and fluorescence imaging systems.

1. Fluorescent Tracing Imaging

LDS 765 is frequently employed as a fluorescent tracer in optical imaging experiments to visualize labeled targets in complex biological or material samples. Researchers use LDS 765-based conjugates to follow distribution, uptake, or localization patterns in fluorescence microscopy and fluorescence imaging setups, benefiting from its far-red emission that helps separate signal from common cellular autofluorescence. This dye is also used when experiments require imaging with standard fluorescence cameras and filter sets compatible with red-channel detection.

2. Gel And Electrophoresis Labeling

LDS 765 is commonly incorporated into fluorescence labeling workflows for protein and biomolecule visualization following electrophoretic separation. In typical research settings, laboratories use LDS 765-containing staining reagents or dye-labeled conjugates to enable sensitive visualization of separated bands in gel-based assays, supporting protein profiling and comparative expression studies. The far-red spectral characteristics are particularly useful for multiplexing or for minimizing interference from background dyes when imaging gels on fluorescence scanners.

3. Fluorescent Conjugate Preparation

LDS 765 is used as a fluorophore component for preparing labeled biomolecules and imaging reagents that can be detected by fluorescence readout. Chemical biology and biomaterials groups incorporate LDS 765 into conjugation strategies to generate fluorescent probes for downstream imaging, localization studies, and assay development. This dye is especially valuable when the labeled conjugate must emit in the red spectral region to improve compatibility with common fluorescence instrumentation and to support imaging in samples with substantial intrinsic fluorescence.

4. Microscopy Based Localization Studies

LDS 765-labeled constructs are applied in fluorescence microscopy to map the spatial distribution of tagged biomolecules or labeled material domains at the cellular or subcellular scale. Imaging teams use LDS 765 when they need a red-emitting fluorophore that performs well in fluorescence channels where background is reduced relative to green/blue labels. The dye's emission profile supports visualization on optical microscopes equipped with appropriate far-red filter sets for cellular imaging and fluorescence localization experiments.