JC-1 | CAS 3520-43-2

Catalog Number	A16-0011
Category	Mitochondrial Fluorescent Probes
Molecular Formula	C25H27Cl4IN4
Molecular Weight	652.2

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Product Introduction

JC-1 is a cyanine dye used for studying mitochondrial integrity in the context of cellular apoptosis. JC-1 exhibits green fluorescence at low membrane potential and aggregates to exhibit red fluorescence at higher membrane potentials.

Chemical Information
Product Specification
Application

Synonyms	5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-1H-benzimidazolium, monoiodide; CBIC2; NK 1420
Purity	≥90%
Canonical SMILES	CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]
InChI	InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
InChIKey	FYNNIUVBDKICAX-UHFFFAOYSA-M
Appearance	Solid Powder

Excitation	485 nm (monomer), 520-570 nm (aggregate)
Emission	535 nm (monomer), 570-610 nm (aggregate)
Storage	Store at -20°C

JC-1, a prominent fluorescent dye in bioscience research, plays a pivotal role in evaluating mitochondrial membrane potential. Here are four key applications of JC-1:

Mitochondrial Health Assessment: Extensively utilized for probing mitochondrial health and activity, JC-1 allows researchers to scrutinize the condition of mitochondria within cells. When mitochondria thrive, JC-1 forms aggregates in the mitochondrial matrix, emitting a vibrant red fluorescence. Conversely, damaged or depolarized mitochondria retain JC-1 in its monomeric state, resulting in a distinct green fluorescence that serves as a reliable indicator of mitochondrial status.

Apoptosis Detection: A critical tool for discerning cell apoptosis, JC-1 aids in tracking changes in mitochondrial membrane potential. As cells undergo early apoptosis, the mitochondrial membrane potential diminishes, causing JC-1 fluorescence to transition from red to green. This shift in fluorescence enables researchers to quantify and monitor apoptotic processes across different cell types under varied experimental settings, offering valuable insights into cell death mechanisms.

Cell Viability Studies: In the realm of cell viability assessments, JC-1 emerges as a key player in evaluating the impact of diverse treatments on mitochondrial function. By analyzing alterations in fluorescence patterns, scientists can gauge how different drugs, toxins, or treatments influence mitochondrial health, and subsequently cell viability. This application holds particular significance in drug screening endeavors and toxicity evaluations, shedding light on the effects of various interventions on cellular well-being.

Cancer Research: Delving into the intricate dynamics of cancer cells, JC-1 finds extensive utility in investigating mitochondrial bioenergetics and dynamics in cancer research. Changes in mitochondrial membrane potential often reflect the metabolic status of cancer cells and their responsiveness to therapeutic interventions. Through JC-1 staining, researchers gain crucial insights into how cancer cells adapt their metabolic pathways, aiding in the identification of potential therapeutic targets and enhancing our understanding of cancer cell behavior.