IR 143 | CAS 54849-65-9

IR 143 is a fluorescent dye used as a long-wavelength imaging label in biochemical and materials research workflows. In practice, IR 143 is selected when red/near-infrared channel compatibility and robust dye conjugation chemistry are needed for tracking, quantification, and visualization. Its strong utility comes from enabling fluorescence readouts in instrumentation setups that support longer-wavelength excitation and emission, including fluorescence microscopy and fluorescence-based assays.

1. Fluorescence Labeling Conjugates

IR 143 is commonly incorporated into fluorescent labeling strategies for proteins, peptides, and other biomolecules where long-wavelength detection improves multiplexing flexibility and reduces interference from shorter-wavelength background. Researchers use IR 143-conjugated biomolecules as imaging reagents for localization studies, binding/interaction experiments, and quantitative fluorescence readouts in assay formats compatible with red/NIR fluorescence detection. In many workflows, IR 143 labeling is chosen to generate stable fluorescence tags for downstream analysis by plate readers, gel-based fluorescence imaging, or microscope-based tracking.

2. Fluorescence Microscopy Imaging

IR 143 supports fluorescence microscopy applications that benefit from longer-wavelength imaging channels, including cellular and material-surface visualization experiments where spectral separation from common autofluorescence is advantageous. Lab teams use IR 143-labeled probes or conjugates to map distribution patterns, monitor uptake or association with prepared substrates, and visualize labeled components in multi-color imaging experiments. When designing microscopy experiments, investigators typically pair IR 143 with appropriate filter sets and excitation sources to ensure efficient excitation and clear emission collection in the selected imaging channel.

3. Flow Cytometry Fluorescent Tagging

IR 143 is used as a fluorescence tag in flow cytometry workflows that require a red/NIR detection channel for analyzing labeled cells or cell-associated reagents. Researchers employ IR 143-conjugated staining reagents to quantify fluorescence intensity distributions for populations exposed to labeled biomolecular constructs, affinity reagents, or surface-associated labeling schemes. In these applications, IR 143's longer-wavelength emission is leveraged to improve channel separation in multi-parameter panels and to support consistent signal detection on standard flow cytometry platforms configured for the relevant spectral range.

4. Fluorescent Bioassay Readouts

IR 143 is frequently used in fluorescence-based bioassay development where a longer-wavelength dye provides a convenient optical reporter for binding, labeling, or interaction readouts. Assay developers incorporate IR 143 into labeled reagents used in plate-based formats, enabling endpoint fluorescence quantification for screening and characterization of biomolecular systems. This includes applications such as fluorescence readout assays for reagent performance evaluation, comparative binding studies, and fluorescence tracing experiments in which the dye's emission is measured using compatible excitation/emission settings.