IR 140 Tosylate

IR 140 Tosylate is a reactive near-infrared cyanine dye tosylate salt designed for covalent fluorescent labeling in chemical biology and materials research. Its electrophilic dye form enables attachment to nucleophiles under appropriate labeling conditions, supporting robust fluorescence imaging workflows with reduced background from visible autofluorescence. Researchers commonly use IR 140 derivatives as far-red/near-IR reporters for conjugate tracking, optical quantification, and fluorescence-based characterization of biomolecule- and polymer-based materials.

1. Antibody And Protein Labeling

IR 140 Tosylate is used by immunochemistry and protein analytics groups to generate near-infrared fluorescent antibody conjugates and protein labeling reagents for imaging and quantitative assays. In these workflows, the dye is covalently installed onto protein scaffolds to produce conjugates that can be tracked during binding studies, wash steps, and comparative imaging experiments. The far-red emission is particularly valued for multiplexing and for experiments where visible-channel background from biological matrices can obscure signal.

2. Peptide And Biomolecule Conjugation

IR 140 Tosylate is applied in bioconjugation workflows that require fluorescent tagging of peptides, affinity ligands, and other biomolecules used as imaging handles or analytical tracers. By installing the dye onto biomolecule platforms, researchers create fluorescent conjugates for studying distribution, retention, and relative abundance in binding and uptake experiments. This dye format is also commonly incorporated into custom fluorescent standards and reagent panels used in optical characterization of biomolecular constructs.

3. Polymer And Biomaterial Surface Labeling

IR 140 Tosylate supports fluorescence labeling of polymers, hydrogels, and biomaterial surfaces used in materials science and biomaterials development. Groups developing functionalized matrices and engineered interfaces use the dye to visualize coating uniformity, assess surface association, and monitor material-related processes by near-infrared fluorescence readouts. Covalent attachment helps maintain signal during washing and handling steps that are typical in microscopy and plate-based fluorescence measurements.

4. Fluorescence Imaging Tracers

IR 140 Tosylate is used to prepare near-infrared imaging tracers for optical tracking in microscopy and fluorescence-based characterization of labeled samples. Researchers incorporate IR 140-labeled conjugates or materials into experimental pipelines where sensitive far-red detection improves contrast against autofluorescence and supports quantitative comparisons across conditions. This makes the dye useful for mapping labeled species in complex experimental environments, including multi-component mixtures and labeled composite materials.