Hydroxystilbamidine bis(methanesulfonate) | CAS 223769-64-0

Hydroxystilbamidine bis(methanesulfonate) is a dicationic bis-methanesulfonate salt of a hydroxystilbene-derived DNA-intercalating dye, widely used in fluorescence-based nucleic acid visualization and analysis. Its strong affinity for nucleic acid structures and bright fluorescence readout make it a practical staining reagent for electrophoresis gels and nucleic acid imaging workflows, where signal localization to DNA enables straightforward band and fragment visualization.

1. DNA Gel Electrophoresis Staining

Hydroxystilbamidine bis(methanesulfonate) is used as a fluorescent stain for post-staining or in-gel visualization of DNA separated by agarose or polyacrylamide electrophoresis. Researchers and core facilities employ it to visualize plasmid DNA, PCR products, and restriction fragments with a workflow that integrates directly with standard gel documentation systems. The dye's nucleic-acid binding behavior supports clear band localization, helping users assess fragment size distributions and confirm amplification or digestion outcomes during routine molecular biology and assay development.

2. Nucleic Acid Fragment Visualization

Hydroxystilbamidine bis(methanesulfonate) supports fluorescence imaging of DNA in formats beyond conventional gels, including microfluidic electrophoresis and nucleic acid fragment tracking in analytical workflows. Molecular biology groups use the stain to monitor DNA integrity and relative abundance when samples are resolved on electrophoretic platforms and imaged with fluorescence-capable instrumentation. In these applications, the dye's DNA-targeted fluorescence provides a convenient readout for comparing sample quality across preparation batches and experimental conditions.

3. Fluorescence Microscopy DNA Localization

Hydroxystilbamidine bis(methanesulfonate) is applied in fluorescence microscopy workflows where DNA localization within fixed or permeabilized samples is required. Cell biology and molecular imaging laboratories use it to visualize nuclei and DNA-containing structures in conjunction with other fluorescent labels, enabling spatial context for nucleic acid distribution in microscopy studies. Its DNA-binding staining behavior makes it useful for mapping nuclear morphology, assessing chromatin organization in fixed preparations, and supporting correlative imaging experiments where DNA serves as a structural reference channel.

4. Flow Cytometry DNA Content Analysis

Hydroxystilbamidine bis(methanesulfonate) is used in fluorescence-based cytometry workflows to report relative DNA content in prepared cell populations. Flow cytometry users incorporate the dye into staining protocols for DNA quantification readouts that support cell-cycle distribution studies and sample characterization in research settings. The dye's nucleic-acid affinity and fluorescence response enable DNA-dependent signal generation that can be analyzed alongside forward/side scatter and additional fluorescence channels for multiplexed sample profiling.