Fluorescein-5-thiosemicarbazide hydrochloride

Catalog Number	A16-0021
Category	Other Cell Fluorescent Probes
Molecular Formula	C21H15N3O5S·HCl
Molecular Weight	457.9

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Product Introduction

Fluorescein-5-thiosemicarbazide hydrochloride is a thiol-reactive fluorophore known for its vibrant green fluorescence, which arises from the fluorescein chromophore class. This compound features a thiosemicarbazide group, enabling it to form covalent bonds with aldehydes and ketones, making it suitable for labeling biomolecules in various fluorescence microscopy and flow cytometry applications. Its excitation and emission properties are leveraged in the development of fluorescence-based assays and biosensing techniques, where it functions as a reporter molecule to track molecular interactions and cellular processes.

Chemical Information
Product Specification

Chemical Information

Related CAS	76863-28-0 (free base)
Synonyms	FTSC; 5-[(hydrazinylthioxomethyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid hydrochloride
Purity	≥95%
SMILES	C1=CC(=C(C=C1NC(=S)NN)C(=O)O)C2=C3C=CC(=O)C=C3OC4=C2C=CC(=C4)O.Cl
InChI	InChI=1S/C21H15N3O5S.ClH/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19;/h1-9,25H,22H2,(H,27,28)(H2,23,24,30);1H
InChIKey	DLAUYFRYXUEQQE-UHFFFAOYSA-N
Appearance	Solid Powder