DAPI

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DAPI

DAPI

DAPI
Catalog Number Size Price Quantity
A16-0201 -- $--

DAPI is commonly used as a nuclear counterstain for fluorescence microscopy, flow cytometry, and chromosome staining. Due to its high affinity for DNA, DAPi is often used in cell counting, apoptosis detection, mycoplasma contamination detection, cell sorting based on DNA content, and nuclear segmentation tools in high-content imaging analysis. Although DAPI can enter living cells at high concentrations, DAPI does not have cell membrane permeability and is usually used to stain fixed cells. For live cell staining, Hoechst 33342 is a popular nuclear counterstain with cell membrane permeability.

Chemical Information
Catelog Number: A16-0201
Product Name: DAPI
CAS Number: 28718-90-3
Molecular Formula: C16H15N5 · 2HCl
Molecular Weight: 350.25
Purity: ≥90%
Synonyms: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI dihydrochloride
Solubility: H2O
MDL Number: MFCD00042988
Product Specification
Melting Point: ≥300 °C
Appearance: Powder
Refractive index: n20/D 1.498 (lit.)
Density: 0.921 g/mL at 25 °C (lit.)
Properties Quality Level: 300
Excitation Maximum (nm): 340
Emission Maximum (nm): 488
Storage Condition: −20°C
Application: Suitable for DNA staining in agarose gels, analysis of changes in DNA during apoptosis, detection of mycoplasma, photofootprinting of DNA, immunofluorescent staining of cells.

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