DAPI dihydrochloride | 28718-90-3
Catelog Number | A16-0201 |
Category | Nuclear Fluorescent Probes |
Molecular Formula | C16H15N5·2HCl |
Molecular Weight | 350.3 |
Catalog Number | Size | Price | Quantity |
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A16-0201 | 250 mg | $439 |
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Product Introduction
DAPI is a fluorescent DNA stain that binds to the minor groove of double-stranded DNA with preference for adenine and thymine (AT) rich DNA. DAPI is commonly used in microscopy and flow cytometry. It can be used on either fixed or live cells, although it crosses membranes in live cells less efficiently and demands higher concentrations to be used.
Chemical Information |
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Related CAS | 47165-04-8 (free base) |
Synonyms | DAPI;2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide dihydrochloride |
Purity | ≥90% |
Canonical SMILES | C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl |
InChI | InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H |
InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
Solubility | H2O |
Density | 0.921 g/mL at 25 °C (lit.) |
Appearance | Yellow Solid |
Refractive Index | n20/D 1.498 (lit.) |
Melting Point | >300°C (dec.) |
MDL Number | MFCD00042988 |
NACRES | NA.52 |
Product Specification |
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Excitation | 358 nm |
Emission | 461 nm |
Properties Quality Level | 300 |
Storage | Store at -20°C |
Application | Suitable for DNA staining in agarose gels, analysis of changes in DNA during apoptosis, detection of mycoplasma, photofootprinting of DNA, immunofluorescent staining of cells. |
Applications of Fluorescent Probes & Dyes
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