Coumarin 461 | 87-01-4

Coumarin 461, a versatile fluorescent dye, finds widespread application in diverse scientific fields, particularly in bioimaging and chemical analysis. Here are key applications of Coumarin 461:

Fluorescence Microscopy: As a fluorescent probe in microscopy, Coumarin 461 plays a pivotal role in visualizing intricate cellular structures and dynamic processes. Its vivid fluorescence and robust stability render it an ideal choice for imaging tasks ranging from tracking internal cellular machinations to monitoring protein localization. Researchers harness the power of Coumarin 461 to delve deep into the realms of cell biology and pathology, unraveling the mysteries of life at the microscopic level.

Flow Cytometry: In the realm of flow cytometry, Coumarin 461 emerges as a beacon, serving as a fluorescent tag to delineate and identify specific cell populations based on their unique fluorescence signatures. This capability enables the comprehensive analysis of cell characteristics, including size, complexity, and marker expression within vast cell samples. Coumarin 461's distinctive emission spectrum empowers its utilization in multi-colored assays, amplifying the capacity for in-depth cellular investigations.

Protein and Nucleic Acid Labeling: The coupling of Coumarin 461 with proteins and nucleic acids opens up a realm of possibilities for investigating molecular interactions and dynamics. By tagging biomolecules with Coumarin 461, researchers can meticulously track their behavior during critical biological processes like protein-protein interactions, DNA hybridization, and enzymatic activities. This application stands as a cornerstone for unraveling the intricate molecular mechanisms that underpin a plethora of biological functions, illuminating the pathways of life's fundamental processes.