Coumarin 445 | 28821-18-3

Coumarin 445 is a fluorescent dye known for its bright blue emission, which finds applications in various scientific fields. Here are some key applications of Coumarin 445:

Fluorescence Microscopy: Coumarin 445 is widely used in fluorescence microscopy to stain and visualize cellular components and structures. Its bright blue fluorescence allows researchers to distinguish it from other dyes emitting at different wavelengths. This application is essential in cell biology for studying cell morphology, intracellular processes, and the localization of specific molecules.

Flow Cytometry: In flow cytometry, Coumarin 445 is employed to label cells or particles for their detection and analysis. Its distinct fluorescence helps in the identification and quantification of different cell populations based on specific markers. This technique is crucial for immunology, cancer research, and diagnosis, as it enables the analysis of cell surface markers, cell cycle, and apoptosis.

Analytical Chemistry: Coumarin 445 serves as a fluorescent probe in various analytical chemistry applications. It is used for the detection and quantification of ions, small molecules, and environmental pollutants in samples. Due to its high sensitivity and specificity, Coumarin 445-based assays help in monitoring chemical reactions, detecting contaminants, and ensuring quality control in various industries.

Biomedical Imaging: Coumarin 445 is utilized in biomedical imaging to trace and study biological processes in living organisms. Its fluorescent properties enable non-invasive imaging of tissues and organs, aiding in the visualization of drug delivery, disease progression, and therapeutic interventions. This application helps researchers and clinicians to gain deeper insights into physiological and pathological conditions.