Ac-WLA-AMC | 1104011-59-7

Ac-WLA-AMC, a synthetic peptide substrate, finds its primary usage in enzyme activity assays and research. Here are four key applications of Ac-WLA-AMC:

Enzyme Activity Assays: Acting as a pivotal tool in the realm of protease activity measurements, Ac-WLA-AMC stands out by functioning as a substrate that liberates a fluorescent signal upon cleavage. This facilitates the straightforward quantification of enzyme activity in vitro under diverse experimental settings. Its extensive utilization in drug discovery is paramount, particularly in the screening of potential inhibitors or activators targeting proteolytic enzymes.

Protease Characterization: Scientists harness the power of Ac-WLA-AMC to delve into the nuances of proteases, unraveling their specificity and kinetics. By closely monitoring the fluorescence rise rate, researchers decipher the affinity and catalytic efficiency of various proteases towards this substrate. This critical information not only sheds light on enzyme functionality but also aids in the development of precise enzyme inhibitors, pushing boundaries of enzymology research.

Biomedical Research: As a cornerstone in the exploration of disease-related proteases, Ac-WLA-AMC plays a pivotal role in dissecting the involvement of these proteases in pathological processes like cancer, inflammation, and neurodegeneration. Researchers leverage this substrate to uncover the biological significance of these proteases and pinpoint potential therapeutic targets. This endeavor contributes significantly to the identification of diagnostic markers and the formulation of effective therapeutic strategies.

High-Throughput Screening: Amidst the landscape of high-throughput screening, Ac-WLA-AMC emerges as a game-changer, enabling rapid and efficient identification of compounds that modulate protease activity. The substrate’s unique ability to generate a quantifiable fluorescent signal lends itself to the automation and scalability of screening processes. This application is indispensable for pharmaceutical entities in their quest to unearth novel drug candidates or optimize enzyme-driven reactions, driving innovation in drug discovery.