4-(4-(Didecylamino)styryl)-N-methylpyridinium iodide | CAS 95378-73-7

4-(4-(Didecylamino)styryl)-N-methylpyridinium iodide, commonly known as DISC3(5), is a versatile fluorescent dye utilized in various biotechnological realms. Here are four key applications:

Membrane Potential Measurement: Enabling the real-time monitoring of changes in membrane potential across cell membranes, this dye plays a pivotal role in cellular physiology. By exhibiting fluorescence in response to voltage fluctuations, it facilitates the in-depth examination of neuronal activity and cardiac function. This application is essential for unraveling the intricate workings of electrophysiological processes and conducting precise drug-screening assays.

Microbial Viability Assays: Applied in the evaluation of microbial cells' viability and vitality, DISC3(5) stands out for its ability to probe cell membranes and respond to electrochemical gradients. This unique characteristic allows for the discrimination between live and deceased cells, making it an indispensable component of microbiological studies and antibiotic susceptibility testing.

Cellular Imaging: Within fluorescence microscopy, this compound serves as a potent tool for visualizing and analyzing cellular structures and functions. Through its ability to stain specific cellular components, it facilitates high-resolution imaging and quantitative analysis. Researchers leverage this capability to delve into cellular interactions, pathway analysis, and structural biology, enhancing our understanding of cellular dynamics.

Apoptosis Detection: In the realm of programmed cell death research, DISC3(5) emerges as a valuable apoptosis marker. With the capacity to detect changes in membrane potential associated with early apoptosis stages, this dye aids in identifying these crucial alterations. This application plays a critical role in cancer research and drug development, where insights into cell death mechanisms are paramount for advancing therapeutic strategies.