YOYO-3 | 156312-20-8

YOYO-3, a fluorescent dye widely utilized in molecular and cellular biology, finds its application in various areas. Here are four key applications of YOYO-3:

Flow Cytometry: Integral to flow cytometry, YOYO-3 serves as a fluorescent label for the precise analysis of DNA content within cells. Its intense fluorescence enables the differentiation of distinct cell populations based on their DNA content, facilitating in-depth studies on cell cycle dynamics, apoptosis, and other cellular phenomena.

Fluorescence Microscopy: In the realm of fluorescence microscopy, YOYO-3 emerges as a vital component for staining cells or tissues to capture detailed images. Its robust binding to double-stranded DNA generates vivid images of the nucleus and chromosomal structures, enabling researchers to visualize cellular morphology and track nuclear alterations throughout various biological processes.

Gel Electrophoresis: YOYO-3 plays a pivotal role in gel electrophoresis, offering a reliable means to visualize DNA fragments. Its fluorescent properties enable the detection of nucleic acids within agarose or polyacrylamide gels under UV or blue light, a critical step in confirming the presence and sizes of DNA fragments in molecular cloning, PCR, and other genetic experiments.

Flow Cytometry: Employed once again in flow cytometry, YOYO-3 functions as a vital tool for labeling and assessing DNA content in cells with its high fluorescence intensity. By facilitating accurate discrimination between different cell populations based on DNA content, this application proves invaluable for investigating cell cycle dynamics, apoptosis, and various cellular processes.