RH 795 | CAS 172807-13-5

RH 795 is a red-emitting fluorescent dye designed for labeling and fluorescence-based readouts in chemical biology and materials workflows. Its strong visible emission supports microscopy and fluorescence instrumentation where robust signal generation is required, and its dye format is commonly leveraged to track biomolecular conjugates, polymeric materials, and labeled assay components. In practical use, RH 795 is selected when a red spectral channel is advantageous for multicolor experiments and when a straightforward fluorescent tag is needed for visualization or quantification.

1. Fluorescence Microscopy Labeling

RH 795 is frequently used as a fluorescent labeling reagent for cellular and biomolecular imaging workflows where red-channel detection improves contrast against background autofluorescence. Researchers incorporate RH 795 into labeled constructs such as stained proteins, polymer-bound materials, or dye-tagged affinity reagents to visualize distribution patterns by epifluorescence and standard confocal microscopy. In multicolor imaging experiments, RH 795 is commonly chosen as a spectrally separated reporter to reduce channel cross-talk and to enable colocalization studies with other fluorophores.

2. Flow Cytometry Fluorescent Tagging

RH 795 supports fluorescence-activated workflows in which a red-emitting dye is used to quantify labeled populations by flow cytometry. In typical use, RH 795 is integrated into fluorescent conjugates for cell labeling experiments, including tracking of dye-tagged biomolecules or labeled binding reagents during sample preparation and analysis. The dye's visible emission enables gating strategies that discriminate RH 795-positive events from unlabeled controls, supporting routine screening and comparative quantification in fluorescence-based assays.

3. Polymer And Biomaterial Staining

RH 795 is applied in biomaterials research to visualize and quantify dye-associated polymer and surface-modified materials. Materials scientists use RH 795 to stain or incorporate fluorescent signal into hydrogels, coatings, and other polymeric platforms to monitor distribution, retention, and surface coverage during fabrication and characterization. This use is also common in studies where fluorescent labeling provides a practical readout for material processing steps, such as comparing batches, evaluating uniformity, or tracking labeled components within composite constructs.

4. Fluorescence Bioassay Development

RH 795 is used as a fluorescent reporter in fluorescence-based assay development where a red-emitting dye provides a convenient measurement signal. Assay developers incorporate RH 795 into labeled assay components, including fluorescent standards, dye-tagged reagents, and calibration materials used to validate assay workflows and instrument settings. In plate-based fluorescence readouts, RH 795 enables straightforward signal generation for monitoring binding or labeling outcomes in research settings that require consistent, visible-wavelength detection.