Resorufin butyrate | 15585-42-9

Resorufin butyrate, a fluorescent substrate utilized in various biochemical assays, finds diverse applications across different fields. Here are four key applications of Resorufin butyrate:

Enzyme Activity Assays: Widely employed in measuring esterase activity in biological samples, Resorufin butyrate undergoes hydrolysis by esterases to yield resorufin, a fluorescent product easily quantifiable. This method offers a sensitive and accurate approach to monitoring enzyme kinetics and screening for inhibitors or activators in drug discovery, illuminating the intricate dynamics of enzymatic processes.

Cell Viability Assays: In the realm of cell viability studies, Resorufin butyrate serves as an invaluable tool for evaluating cellular health and proliferation. Metabolically active cells enzymatically process Resorufin butyrate into resorufin, generating a fluorescent signal that mirrors cell number and viability. This application proves particularly effective in assessing compound-induced cytotoxic effects and facilitating high-throughput screening initiatives, enriching our understanding of cellular responses to various stimuli.

Tissue Imaging: Delving into histological investigations, Resorufin butyrate enables the visualization of esterase activity within tissue sections through the emission of a fluorescent signal upon hydrolysis. This imaging technique aids in precisely localizing and quantifying enzyme activity in specific tissues or cellular compartments, offering insights into tissue-specific enzyme expression patterns and enzymatic activities, thus enhancing our comprehension of tissue microenvironments.

Biodegradation Studies: Within the realm of environmental science, Resorufin butyrate emerges as a valuable tool for probing the microbial degradation of ester-containing pollutants. Microorganisms equipped with esterases can metabolize Resorufin butyrate into resorufin, yielding a fluorescent signal that serves as a readout for biodegradation processes. This application plays a pivotal role in monitoring and assessing the efficacy of bioremediation strategies, shedding light on the complex interplay between microorganisms and environmental pollutants in a dynamic and visually striking manner.