Sulfo-Cyanine5-PEG3-Biotin

Product Introduction

Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.,Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.,Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.,The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.

Chemical Information
Purity	NMR 1H, HPLC-MS (95%)
IUPAC Name	potassium;1-[6-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethylamino]-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
Canonical SMILES	CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)NCCOCCOCCNC(=O)CCCCC5C6C(CS5)NC(=O)N6)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C.[K+]
InChI	InChI=1S/C48H66N6O11S3.K/c1-47(2)35-30-33(67(58,59)60)19-21-38(35)53(5)41(47)15-8-6-9-16-42-48(3,4)36-31-34(68(61,62)63)20-22-39(36)54(42)25-13-7-10-17-43(55)49-23-26-64-28-29-65-27-24-50-44(56)18-12-11-14-40-45-37(32-66-40)51-46(57)52-45;/h6,8-9,15-16,19-22,30-31,37,40,45H,7,10-14,17-18,23-29,32H2,1-5H3,(H5-,49,50,51,52,55,56,57,58,59,60,61,62,63);/q;+1/p-1/t37-,40-,45-;/m0./s1
InChI Key	AFUPYAGXPYBXDE-DHAJKTQTSA-M
Solubility	Good in water, DMF, DMSO
Appearance	Dark Blue solid

Product Specification
CF260	0.04
CF280	0.04
Fluorescence Quantum Yield	0.28
Excitation	646
Emission	662
Storage	Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Computed Properties
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	13
Rotatable Bond Count	23
Exact Mass	1036.35105192 g/mol
Monoisotopic Mass	1036.35105192 g/mol
Topological Polar Surface Area	281Ų
Heavy Atom Count	69
Formal Charge	0
Complexity	2090
Isotope Atom Count	0
Defined Atom Stereocenter Count	3
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	3
Covalently-Bonded Unit Count	2
Compound Is Canonicalized	Yes