N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 | CAS 2107273-72-1

N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 is a PEGylated Cy5 fluorescent dye designed to improve aqueous handling while retaining the long-wavelength Cy5 emission commonly used for fluorescence imaging workflows. Its amide-bearing PEG architecture supports conjugate construction and formulation for labeling studies where reduced nonspecific interactions and flexible linker spacing are beneficial.

1. Fluorescent Protein Labeling

N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 is used in fluorescence labeling of proteins and protein-derived reagents in chemical biology and biomaterials laboratories. Researchers incorporate the Cy5 fluorophore into protein conjugates to enable visualization of binding, trafficking, or immobilization events during assay development and imaging experiments, taking advantage of the dye's far-red emission to reduce interference from shorter-wavelength fluorophores.

2. Biomaterial Surface Labeling

N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 is applied to label polymeric and hydrogel-based materials for tracking surface association and coating uniformity. In materials science and interface chemistry workflows, the PEGylated Cy5 scaffold helps produce stable, water-compatible fluorescent coatings that can be imaged by fluorescence microscopy to evaluate adsorption behavior, surface coverage, and spatial distribution on engineered substrates.

3. Fluorescence Imaging Tracing

N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 supports fluorescence imaging tracing experiments where long-wavelength Cy5 readout is preferred for background reduction. Scientists use Cy5-labeled constructs as molecular tracers to follow transport or localization in labeled samples, including studies that require flexible PEG linkers for improved dispersion and reduced aggregation during sample preparation and imaging.

4. Conjugate Building Block

N-(m-PEG4)-N'-(4-hydroxycyclohexyl-1-amido-PEG4)-Cy5 is frequently selected as a fluorescent building block for constructing custom labeling reagents and probe-like conjugates. In research settings focused on reagent development, the PEGylated Cy5 design provides a convenient fluorescent handle for integrating into larger molecular assemblies, enabling downstream use in fluorescence-based characterization of conjugates, binding partners, or labeled materials.