Mca-YVADAP-Lys(Dnp)-OH

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Mca-YVADAP-Lys(Dnp)-OH

Mca-YVADAP-Lys(Dnp)-OH | 189696-01-3

Catalog Number A18-0044
Category Fluorescent Enzyme Substrates
Molecular Formula C53H64N10O19
Molecular Weight 1145.15
Catalog Number Size Price Quantity
A18-0044 -- $--

Product Introduction

Mca-YVADAP-Lys(Dnp)-OH is a fluorogenic substrate for caspase-1 and angiotensin-converting enzyme 2 (ACE2). It is cleaved by caspase-1 or ACE2 to release the fluorescent moiety 7-methoxycoumarin-4-acetyl (Mca), which can be used to quantify enzyme activity.

Chemical Information

Synonyms Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH; Caspase-1 Fluorogenic Substrate V; N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-tyrosyl-L-valyl-L-alanyl-L-α-aspartyl-L-alanyl-L-prolyl-N6-(2,4-dinitrophenyl)-L-lysine; L-Lysine, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-tyrosyl-L-valyl-L-alanyl-L-alpha-aspartyl-L-alanyl-L-prolyl-N6-(2,4-dinitrophenyl)-
Purity ≥95%
IUPAC Name (2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-3-carboxy-2-[[(2S)-2-[[(2S)-2-[[(2S)-3-(4-hydroxyphenyl)-2-[[2-(7-methoxy-2-oxochromen-4-yl)acetyl]amino]propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]propanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-6-(2,4-dinitroanilino)hexanoic acid
Canonical SMILES CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(CCCCNC2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-])C(=O)O)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)CC4=CC(=O)OC5=C4C=CC(=C5)OC
InChI InChI=1S/C53H64N10O19/c1-27(2)46(60-49(71)38(21-30-11-14-33(64)15-12-30)57-43(65)22-31-23-45(68)82-42-25-34(81-5)16-17-35(31)42)51(73)55-28(3)47(69)59-39(26-44(66)67)48(70)56-29(4)52(74)61-20-8-10-40(61)50(72)58-37(53(75)76)9-6-7-19-54-36-18-13-32(62(77)78)24-41(36)63(79)80/h11-18,23-25,27-29,37-40,46,54,64H,6-10,19-22,26H2,1-5H3,(H,55,73)(H,56,70)(H,57,65)(H,58,72)(H,59,69)(H,60,71)(H,66,67)(H,75,76)/t28-,29-,37-,38-,39-,40-,46-/m0/s1
InChI Key XLXKHBNLWUCNOC-XUFBPUFYSA-N
Solubility Soluble in DMSO
Density 1.4±0.1 g/cm3
Appearance Yellow Powder
Boiling Point 1507.2±65.0°C at 760 mmHg
  • Product Specification
  • Application
Storage Store at -20°C

Mca-YVADAP-Lys(Dnp)-OH serves as a valuable fluorescent substrate for studying caspase activity, particularly caspase-1 and caspase-3. By incorporating the Mca (7-methoxycoumarin-4-acetic acid) fluorophore, this substrate provides a reliable and sensitive means to detect caspase-mediated cleavage events in various biological samples. Researchers utilize this substrate to monitor apoptosis and inflammation in cell cultures, allowing for real-time observation of caspase activation and subsequent cellular responses. This application is essential for elucidating the roles of caspases in disease processes such as cancer and neurodegeneration, thereby contributing to the understanding of potential therapeutic targets.

Another significant application of Mca-YVADAP-Lys(Dnp)-OH is in drug discovery and development. By using this substrate in high-throughput screening assays, pharmaceutical companies can identify compounds that selectively inhibit or activate caspases. The fluorescent readout enables rapid assessment of compound effects on caspase activity, facilitating the discovery of novel drug candidates that can modulate apoptosis. This application is particularly relevant in cancer research, where regulating caspase activity may enhance the efficacy of existing therapies or help develop new treatment strategies.

Mca-YVADAP-Lys(Dnp)-OH is also employed in studying inflammatory diseases, where caspase-1 plays a pivotal role in the activation of the inflammasome and the subsequent release of pro-inflammatory cytokines. Researchers use this substrate to investigate the mechanisms underlying inflammatory responses and to screen for potential anti-inflammatory agents. The ability to visualize caspase-1 activity provides insights into the dynamics of inflammation in conditions such as rheumatoid arthritis and sepsis, aiding in the development of targeted therapies that can mitigate inflammatory damage.

Lastly, Mca-YVADAP-Lys(Dnp)-OH is utilized in the field of proteomics, specifically in the identification and quantification of caspase substrates. By employing this fluorescent substrate in combination with mass spectrometry, scientists can decipher the caspase cleavage landscape within complex biological samples. This application is critical for mapping signaling pathways and understanding cellular regulation mechanisms. Identifying specific substrates cleaved by caspases not only furthers our knowledge of cell death pathways but also opens avenues for biomarker discovery in various diseases, enhancing diagnostic capabilities.

Computed Properties

XLogP3 3.3
Hydrogen Bond Donor Count 10
Hydrogen Bond Acceptor Count 20
Rotatable Bond Count 27
Exact Mass 1144.43491985 g/mol
Monoisotopic Mass 1144.43491985 g/mol
Topological Polar Surface Area 429Ų
Heavy Atom Count 82
Formal Charge 0
Complexity 2370
Isotope Atom Count 0
Defined Atom Stereocenter Count 7
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes
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