LysoTracker Red DND-99 | 231946-72-8

Product Introduction

Lysotracker probe is an acidic fluorescent probe, used for labeling and tracing acidic organelles in living cells. These probes have several important features, including highly selective targeting of acidic organelles and nanomolar concentration to effectively label living cells. In addition, the LysoTracker series has several fluorescent colors, which are particularly suitable for multi-color labeling experiments. The Lysotracker probe contains a fluorescein attached to a weak base, which is partially protonated at neutral pH, can freely penetrate the cell membrane and is typically concentrated on spherical organelles. The study found that the Lysotracker probe must be at a very low concentration (usually about 50nM) in order to obtain excellent selectivity. Although the retention mechanism of these probes has not been fully studied, it is likely to be related to the protonation and retention of acidic organelles. Even if the cells are treated with weak alkaline cell permeation compounds, the staining results are usually not reversible. The endocytosis kinetics study of the Lysotracker probe shows that the uptake rate of the dye into living cells is only a few seconds. However, these lysosomal probes can cause lysosomes to be alkalized, for example, long-term incubation can induce an increase in lysosomal pH. Therefore, it is recommended to incubate the cells with the probe for only 1-5min before imaging. LysoTracker Red DND-99 is a red fluorescent dye that stains acidic compartments in living cells, with a maximum excitation/emission wavelength of 577/590 nm. This product is a DMSO storage solution with a concentration of 1 mM. It is used for lysosomal staining of living cells. The recommended working concentration is 50-75 nM, which needs to be optimized and adjusted according to the actual situation.