MHI-148 | CAS 172971-76-5

MHI-148 is a research-grade fluorescent labeling reagent designed for chemical biology workflows where controlled attachment of a fluorophore to biomolecules is required. Its reactive functionality enables coupling to amine- and other nucleophile-containing targets, supporting downstream fluorescence microscopy and assay development. The dye's emission in the visible range makes it practical for standard fluorescence instrumentation used in imaging and quantitative readouts.

1. Biomolecule Fluorescent Labeling

MHI-148 is frequently used by chemical biology and proteomics researchers to generate fluorescently labeled proteins, peptides, and other biomolecules for localization and binding studies. In labeling workflows, the reagent is incorporated into conjugate preparation so that the resulting fluorescent conjugate can be tracked during purification, incubation, and analysis. Because the labeling is compatible with common fluorescence readouts, MHI-148 is often selected when a robust, covalent fluorescent tag is needed for comparative imaging experiments or fluorescence-based binding assays.

2. Fluorescence Microscopy Staining

MHI-148 supports fluorescence microscopy workflows where researchers need a labeled biomolecular component to visualize cellular interactions, uptake-related processes, or extracellular binding events. Teams developing imaging reagents use MHI-148 to prepare fluorescent conjugates that can be introduced into microscopy sample preparation, enabling spatial visualization using standard filter sets matched to the dye's emission. This application is particularly common in studies that require consistent labeling of a target reagent rather than relying on noncovalent adsorption.

3. Flow Cytometry Labeling

MHI-148 is used in flow cytometry reagent development to create fluorescent conjugates for quantitative analysis of labeled biomolecules in cell-based or bead-based formats. In these workflows, the reagent is incorporated during conjugate preparation so that fluorescence intensity reports on the presence of the labeled component after incubation and wash steps. Researchers often choose MHI-148 when they want a covalently labeled probe that can be measured reproducibly across samples using routine flow cytometry instrumentation.

4. Fluorescence-Based Bioassays

MHI-148 is commonly applied in fluorescence assay development where a labeled reagent is used as a measurable reporter for binding or interaction readouts. Assay developers incorporate the dye into conjugates used in plate-based experiments, enabling fluorescence intensity monitoring as an experimental endpoint. This use case is especially relevant for screening and characterization studies that require a stable fluorescent tag on a biomolecule to support consistent assay readouts across multiple experimental conditions.