FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer is a fluorescein (FAM)-labeled dUTP analog designed for enzymatic incorporation of a fluorescent nucleotide into DNA. In standard nucleic acid workflows, the FAM fluorophore provides green fluorescence for visualization and quantification, enabling fluorescent tracking of newly synthesized or incorporated DNA strands in microscopy, plate-based assays, and electrophoretic readouts. Its uracil functionality supports use in polymerase-driven labeling strategies where dUTP analogs are accepted substrates.

1. Fluorescent DNA Labeling

FAM-11-dUTP, 6-isomer is used to generate fluorescently labeled DNA for downstream imaging and analysis, including labeling of PCR products, nick translation-derived probes, and polymerase-based incorporation workflows. Researchers commonly employ it to visualize DNA distribution on gels, membranes, or microscope slides where fluorescein emission provides a convenient readout. Because the label is introduced through nucleotide incorporation, the resulting DNA conjugate retains the native sequence context of the target amplicon or probe, which is valuable for mapping hybridization behavior and tracking DNA handling steps in molecular biology workflows.

2. Nucleic Acid Hybridization Probes

FAM-11-dUTP, 6-isomer supports the preparation of fluorescent DNA probes for hybridization-based assays, including probe generation for blotting workflows and fluorescence readouts of nucleic acid binding. Teams in molecular diagnostics research and nucleic acid analysis laboratories use fluorescein-labeled DNA to monitor hybridization events using fluorescence imaging or plate readers, especially when a DNA probe needs to be produced in a sequence-specific manner. The dUTP analog format enables incorporation into probe DNA using enzymatic synthesis or modification approaches, producing a probe that can be interrogated under hybridization conditions with standard fluorescence detection instrumentation.

3. Fluorescence-Based qPCR Assay Development

FAM-11-dUTP, 6-isomer is used in fluorescence assay development where incorporation of a fluorescein-labeled nucleotide provides a measurable signal that scales with DNA synthesis during amplification. Molecular biology groups developing or optimizing nucleic-acid amplification readouts can use it to create fluorescent templates or to support assay design experiments that rely on fluorescence from incorporated nucleotides rather than only from intercalating dyes. In these workflows, the reagent's nucleotide identity helps integrate the fluorescent label into the amplified DNA, enabling monitoring of amplification progress with compatible fluorescence detection setups.

4. Fluorescent Microscopy DNA Tracing

FAM-11-dUTP, 6-isomer is applied in microscopy-oriented studies that require fluorescent DNA tracing, such as monitoring DNA uptake, trafficking, or localization in fixed sample formats where fluorescein imaging is practical. Researchers use fluorescein-labeled DNA to follow DNA-containing constructs through sample preparation and imaging, leveraging the well-established detection of FAM in standard fluorescence microscope filter sets. This makes the reagent useful for experimental pipelines where DNA needs to be visualized as a fluorescent molecular marker while maintaining sequence-specific identity of the labeled material.