DusQ 21 carboxylic acid | CAS 1200894-41-2

DusQ 21 carboxylic acid is a fluorescence quencher designed for signal suppression in Förster resonance energy transfer (FRET) and fluorescence-based nucleic-acid or probe formats. Its carboxylic acid functionality supports conjugation and incorporation into labeling workflows where controlled quenching is needed to reduce background and enable distance-dependent fluorescence readout.

1. FRET Quencher Labeling

DusQ 21 carboxylic acid is used as a quenching partner in FRET-based probe designs for fluorescence readouts in chemical biology and assay development. Researchers incorporate the quencher into donor-quencher constructs to regulate fluorescence intensity so that emission from a fluorescent reporter is suppressed under defined conditions and recovered upon target-dependent changes in probe geometry or separation.

2. Molecular Beacon Design

DusQ 21 carboxylic acid supports the construction of quenched oligonucleotide probes where fluorescence is held low in the absence of the target and increases upon hybridization-driven structural rearrangement. In nucleic acid analysis workflows, the carboxyl group enables practical conjugation strategies for positioning the quencher on the oligonucleotide, supporting robust signal control for fluorescence microscopy and plate-based fluorescence measurements.

3. Oligonucleotide Probe Conjugation

DusQ 21 carboxylic acid is commonly employed in oligonucleotide labeling workflows that require an effective fluorescence quencher for background reduction in hybridization assays. Molecular imaging and nucleic acid detection developers use the quencher to build reporter-quencher oligos for fluorescence-based readouts, including probe sets used in research-grade genotyping, expression analysis, and nucleic acid binding studies.

4. Biosensor Signal Suppression

DusQ 21 carboxylic acid is applied in fluorescence biosensor development as a quencher element to manage baseline signal from fluorescent reporters in engineered sensing platforms. Teams developing probe-based or affinity-based sensors use the carboxyl-functional quencher to integrate signal suppression into conjugates and materials, enabling fluorescence to reflect binding- or event-dependent changes rather than unstructured background.