DusQ 21 CPG 500

DusQ 21 CPG 500 is a quencher-conjugated controlled pore glass (CPG) support designed for solid-phase fluorescence signal suppression in nucleic acid and probe assembly workflows. By integrating a fluorescence-quenching moiety onto a porous inorganic carrier, this reagent supports background control during oligonucleotide labeling and probe construction, where quencher placement and distance-dependent quenching behavior are critical for fluorescence readouts. Researchers use DusQ 21 CPG 500 in automated solid-phase formats to generate quencher-bearing oligonucleotides and molecular beacon-type constructs for fluorescence-based assays.

1. Molecular Beacon Quenching

DusQ 21 CPG 500 is used to build quencher-containing molecular beacons and related stem-loop oligonucleotide probes where fluorescence is suppressed in the unhybridized state and recovered upon target-dependent conformational change. Solid-phase integration on a porous CPG support helps streamline quencher incorporation during automated oligonucleotide synthesis, supporting consistent quencher placement within the probe architecture for fluorescence imaging and plate-based kinetic measurements.

2. Oligonucleotide Probe Construction

DusQ 21 CPG 500 supports the preparation of quencher-modified oligonucleotide probes used for fluorescence hybridization assays and nucleic-acid workflow development. In these applications, the quencher-bearing support enables researchers to incorporate a defined quenching element into probe designs intended for fluorescence monitoring, including endpoint and time-resolved readouts on common fluorescence plate readers and imaging systems.

3. FRET-Based Assay Building

DusQ 21 CPG 500 is applied in the development of FRET pairs and fluorescence-controlled nucleic acid assays where a quencher element is required to manage donor emission and reduce background. Probe developers commonly incorporate quencher-bearing segments into oligonucleotide scaffolds to create distance-dependent signal modulation, enabling robust fluorescence reporting strategies for assay prototyping and optimization in molecular biology and chemical biology laboratories.

4. Solid-Phase Probe Immobilization

DusQ 21 CPG 500 is also leveraged in workflows that require solid-support-based fluorescence control, including the preparation of quencher-functionalized oligonucleotide materials for downstream assay format engineering. By using a CPG carrier, researchers can integrate quencher functionality into solid-phase constructs that are compatible with automated synthesis and subsequent purification steps, supporting reproducible fluorescence suppression in custom probe platforms.