Cyanine5.5 azide
Catalog Number | F02-0011 |
Category | Cyanine |
Molecular Formula | C43H49ClN6O |
Molecular Weight | 701.34 |
Catalog Number | Size | Price | Quantity |
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F02-0011 | 10 mg | $698 |
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Product Introduction
This Cyanine5.5 labeling reagent is a dye azide for Click Chemistry, available as 10 mM solution in DMSO, or in solid form. The dye possesses far red / near infrared emission, which allows to use it for NIR live organism imaging. Cyanine5.5 can replace Cy5.5®, Alexa Fluor 680, and DyLight 680.
Chemical Information |
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Purity | NMR 1H, HPLC-MS (95%) |
IUPAC Name | N-(3-azidopropyl)-6-[1,1-dimethyl-2-[5-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)penta-2,4-dienylidene]benzo[e]indol-3-yl]hexanamide;chloride |
Canonical SMILES | CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC=CC=C4C(C5=C(N4CCCCCC(=O)NCCCN=[N+]=[N-])C=CC6=CC=CC=C65)(C)C)C.[Cl-] |
InChI | InChI=1S/C43H48N6O.ClH/c1-42(2)37(48(5)35-26-24-31-17-11-13-19-33(31)40(35)42)21-8-6-9-22-38-43(3,4)41-34-20-14-12-18-32(34)25-27-36(41)49(38)30-15-7-10-23-39(50)45-28-16-29-46-47-44;/h6,8-9,11-14,17-22,24-27H,7,10,15-16,23,28-30H2,1-5H3;1H |
InChI Key | KOVQVUIEVDGEDR-UHFFFAOYSA-N |
Solubility | soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (1.6 uM, 1.2 mg/L) |
Appearance | dark blue powder / solution |
- Product Specification
- Application
ε, L⋅mol-1⋅cm-1 | 198000 |
Fluorescence Quantum Yield | 0.2 |
Excitation | 684 |
Emission | 710 |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Cyanine5.5 azide, a vibrant fluorescent dye favored in biological and chemical research, boasts remarkable brightness and stability. Here, we unveil four key applications of Cyanine5.5 azide:
Fluorescence Imaging: As a stalwart in fluorescence imaging, Cyanine5.5 azide is a beacon for labeling and visualizing biological entities like proteins, nucleic acids, and cells. Its robust quantum yield and photostability position it as the go-to choice for enduring imaging endeavors. Researchers rely on this dye to monitor the spatial distribution and dynamic motions of target molecules within live or fixed cells, unraveling intricate biological processes in real-time.
Molecular Probes: Delving into molecular interactions within complex systems, Cyanine5.5 azide emerges as a versatile fluorescent probe, easily conjugated to various biomolecules. This application is instrumental for deciphering the inner workings and mechanisms of biological entities. Leveraging azide-alkyne click chemistry ensures precise targeting of probes with minimal disruption to biological functions, shedding light on elusive molecular events with surgical precision.
Flow Cytometry: In the realm of flow cytometry, Cyanine5.5 azide plays a pivotal role in staining cells and assessing their properties based on fluorescence intensity. It serves as a linchpin in discerning distinct cell populations, pinpointing specific markers, and evaluating cellular functions with laser focus. This capability holds critical importance in expediting high-throughput analyses across immunological, oncological, and biomedical landscapes, guiding groundbreaking research endeavors.
In Vivo Imaging: Breaking new ground in in vivo imaging, Cyanine5.5 azide transcends boundaries in tracking compound distribution and accumulation in animal models. Its near-infrared fluorescence affords deeper tissue penetration and reduced background noise, enabling comprehensive monitoring of disease progression, drug delivery efficiency, and biodistribution patterns. This visionary tool empowers researchers to visualize and interpret dynamic biological processes with unparalleled clarity and depth.
Computed Properties | |
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Hydrogen Bond Donor Count | 1 |
Hydrogen Bond Acceptor Count | 5 |
Rotatable Bond Count | 13 |
Exact Mass | 700.3656379 g/mol |
Monoisotopic Mass | 700.3656379 g/mol |
Topological Polar Surface Area | 49.7Ų |
Heavy Atom Count | 51 |
Formal Charge | 0 |
Complexity | 1370 |
Isotope Atom Count | 0 |
Defined Atom Stereocenter Count | 0 |
Undefined Atom Stereocenter Count | 0 |
Defined Bond Stereocenter Count | 0 |
Undefined Bond Stereocenter Count | 3 |
Covalently-Bonded Unit Count | 2 |
Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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