Cyanine5.5 azide

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Cyanine5.5 azide

Cyanine5.5 azide

Catalog Number F02-0011
Category Cyanine
Molecular Formula C43H49ClN6O
Molecular Weight 701.34
Catalog Number Size Price Quantity
F02-0011 10 mg $698

Product Introduction

This Cyanine5.5 labeling reagent is a dye azide for Click Chemistry, available as 10 mM solution in DMSO, or in solid form. The dye possesses far red / near infrared emission, which allows to use it for NIR live organism imaging. Cyanine5.5 can replace Cy5.5®, Alexa Fluor 680, and DyLight 680.

Chemical Information

Purity NMR 1H, HPLC-MS (95%)
IUPAC Name N-(3-azidopropyl)-6-[1,1-dimethyl-2-[5-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)penta-2,4-dienylidene]benzo[e]indol-3-yl]hexanamide;chloride
Canonical SMILES CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC=CC=C4C(C5=C(N4CCCCCC(=O)NCCCN=[N+]=[N-])C=CC6=CC=CC=C65)(C)C)C.[Cl-]
InChI InChI=1S/C43H48N6O.ClH/c1-42(2)37(48(5)35-26-24-31-17-11-13-19-33(31)40(35)42)21-8-6-9-22-38-43(3,4)41-34-20-14-12-18-32(34)25-27-36(41)49(38)30-15-7-10-23-39(50)45-28-16-29-46-47-44;/h6,8-9,11-14,17-22,24-27H,7,10,15-16,23,28-30H2,1-5H3;1H
InChI Key KOVQVUIEVDGEDR-UHFFFAOYSA-N
Solubility soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (1.6 uM, 1.2 mg/L)
Appearance dark blue powder / solution
  • Product Specification
  • Application
ε, L⋅mol-1⋅cm-1 198000
Fluorescence Quantum Yield 0.2
Excitation 684
Emission 710
Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5.5 azide, a vibrant fluorescent dye favored in biological and chemical research, boasts remarkable brightness and stability. Here, we unveil four key applications of Cyanine5.5 azide:

Fluorescence Imaging: As a stalwart in fluorescence imaging, Cyanine5.5 azide is a beacon for labeling and visualizing biological entities like proteins, nucleic acids, and cells. Its robust quantum yield and photostability position it as the go-to choice for enduring imaging endeavors. Researchers rely on this dye to monitor the spatial distribution and dynamic motions of target molecules within live or fixed cells, unraveling intricate biological processes in real-time.

Molecular Probes: Delving into molecular interactions within complex systems, Cyanine5.5 azide emerges as a versatile fluorescent probe, easily conjugated to various biomolecules. This application is instrumental for deciphering the inner workings and mechanisms of biological entities. Leveraging azide-alkyne click chemistry ensures precise targeting of probes with minimal disruption to biological functions, shedding light on elusive molecular events with surgical precision.

Flow Cytometry: In the realm of flow cytometry, Cyanine5.5 azide plays a pivotal role in staining cells and assessing their properties based on fluorescence intensity. It serves as a linchpin in discerning distinct cell populations, pinpointing specific markers, and evaluating cellular functions with laser focus. This capability holds critical importance in expediting high-throughput analyses across immunological, oncological, and biomedical landscapes, guiding groundbreaking research endeavors.

In Vivo Imaging: Breaking new ground in in vivo imaging, Cyanine5.5 azide transcends boundaries in tracking compound distribution and accumulation in animal models. Its near-infrared fluorescence affords deeper tissue penetration and reduced background noise, enabling comprehensive monitoring of disease progression, drug delivery efficiency, and biodistribution patterns. This visionary tool empowers researchers to visualize and interpret dynamic biological processes with unparalleled clarity and depth.

Computed Properties

Hydrogen Bond Donor Count 1
Hydrogen Bond Acceptor Count 5
Rotatable Bond Count 13
Exact Mass 700.3656379 g/mol
Monoisotopic Mass 700.3656379 g/mol
Topological Polar Surface Area 49.7Ų
Heavy Atom Count 51
Formal Charge 0
Complexity 1370
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 3
Covalently-Bonded Unit Count 2
Compound Is Canonicalized Yes
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