3'-CY5.5 CPG 1000

3'-CY5.5 CPG 1000 is a Cy5.5-labeled controlled pore glass (CPG) support designed for building fluorescently tagged oligonucleotides and related nucleic-acid probes on solid phase. The Cy5.5 fluorophore provides a far-red readout that is commonly leveraged in fluorescence-based nucleic acid analysis, including imaging and assay development where reduced spectral overlap with shorter-wavelength dyes is advantageous. Researchers use this support to generate labeled DNA/RNA constructs with consistent dye loading at the 3′ terminus for downstream hybridization and detection workflows.

1. 3′-End Oligonucleotide Labeling

3'-CY5.5 CPG 1000 is used by nucleic acid chemistry groups to incorporate a Cy5.5 fluorophore at the 3′ end of DNA or RNA during solid-phase synthesis on controlled pore glass. This format supports workflows where the dye position relative to the sequence is important for hybridization behavior, probe accessibility, or fluorescence readout during post-synthesis assays. Typical users include oligonucleotide probe developers for fluorescence hybridization experiments and researchers constructing labeled strands for molecular imaging and nucleic acid binding studies.

2. Fluorescent Hybridization Probes

3'-CY5.5 CPG 1000 enables production of far-red fluorescent oligonucleotide probes used in hybridization-based detection formats. After synthesis, the Cy5.5-labeled 3′-end oligonucleotides can be employed to visualize or quantify nucleic acid targets by fluorescence microscopy, plate-based fluorescence assays, or other nucleic-acid analysis workflows that rely on target-dependent fluorescence. This support is particularly relevant when probe design calls for a defined terminal labeling strategy that supports consistent performance across probe batches.

3. Molecular Imaging Reagent Development

3'-CY5.5 CPG 1000 is applied in the development of Cy5.5-tagged nucleic-acid imaging reagents, where far-red emission is used to improve compatibility with common fluorescence imaging setups and multiplexing schemes. Teams in chemical biology and molecular imaging often use 3′-labeled oligonucleotides to track hybridization events, monitor probe localization in imaging experiments, or generate fluorescent standards for method development. The solid-phase, support-based labeling approach helps researchers generate reproducible labeled constructs for imaging reagent prototyping and optimization.

4. Assay-Ready Fluorescent Standards

3'-CY5.5 CPG 1000 supports the preparation of Cy5.5-labeled oligonucleotides used as reference materials in assay development and method validation. Laboratories developing fluorescence readouts for nucleic acid workflows frequently require well-defined fluorescent oligos to calibrate instrument settings, verify labeling consistency, and benchmark assay conditions across experimental runs. This makes the 3′-terminal Cy5.5 labeling format useful for teams standardizing fluorescence-based nucleic acid analysis protocols.