C11 BODIPY 581/591 | CAS 217075-36-0

C11 BODIPY 581/591 is a BODIPY-family fluorescent dye designed for bright fluorescence in the orange-red spectral region, commonly paired with standard fluorescence optics for labeling and imaging workflows. Its hydrophobic BODIPY core provides strong emission, while the C11 substituent supports use in membrane-associated and lipid-analog staining formats where dye retention and signal localization are important for microscopy and fluorescence-based assays.

1. Membrane Lipid Labeling

C11 BODIPY 581/591 is frequently used as a lipid-associated fluorescence probe for staining and tracking membrane environments in cell biology and biomaterials research. Researchers incorporate the dye into staining protocols to visualize lipid-rich regions and to monitor distribution changes during experimental treatments, since the dye's BODIPY emission in the 580-600 nm range aligns well with common fluorescence filter sets and imaging channels. This reagent is also used when building fluorescence readouts for membrane-related studies, including comparisons of membrane organization across sample conditions.

2. Fluorescence Microscopy Imaging

C11 BODIPY 581/591 supports fluorescence microscopy workflows aimed at cellular imaging and subcellular localization studies where an orange-red emitting fluorophore is advantageous. Imaging teams use the dye to generate high-contrast fluorescence signals for qualitative localization and quantitative image analysis, leveraging its established BODIPY brightness and compatibility with routine confocal and widefield setups. The dye is commonly selected when researchers need a robust fluorescent label for visualization of lipid-associated structures and for co-localization experiments with spectrally separated markers.

3. Flow Cytometry Lipid Staining

C11 BODIPY 581/591 is applied in flow cytometry as a fluorescence staining reagent to quantify lipid-associated signal across large cell populations. In typical cytometry workflows, the dye is used to generate a single-channel or multi-color readout that reflects relative staining intensity, enabling population-level comparisons in imaging-to-flow studies and phenotypic screening experiments that involve membrane or lipid state changes. Its emission band around the 590 nm region fits well with many common cytometer detection configurations for orange-red fluorescence.

4. Fluorescent Assay Development

C11 BODIPY 581/591 is used as a fluorescent reporter in assay development where a stable, bright dye is needed to monitor lipid-related processes or fluorescence-based endpoints. Assay developers incorporate the dye into protocols that rely on fluorescence intensity changes or distribution patterns as the measurable signal, including format optimization for microplate-based readouts and imaging-compatible assays. The dye's BODIPY emission properties make it a practical choice for building robust fluorescence readouts in screening and materials characterization workflows that involve lipid-rich components.