BODIPY 576/589 | CAS 150173-78-7

BODIPY 576/589 is a bright BODIPY-family fluorescent dye engineered for efficient fluorescence in the orange-red spectral region, commonly used as a labeling fluorophore in chemical biology and materials research. Its compact, photostable scaffold and strong emission make it a practical choice for preparing fluorescent conjugates where robust signal reporting is required. The dye's labeling-friendly character supports incorporation into biomolecule conjugates, polymers, and imaging standards for microscopy- and fluorescence-based workflows.

1. Fluorescent Labeling Conjugates

BODIPY 576/589 is widely used as a fluorophore for preparing fluorescent labeling conjugates for proteins, peptides, and other research biomolecules. In typical workflows, researchers incorporate the dye into labeling reagents or conjugates to visualize distribution, monitor binding interactions, or track biomolecule localization in solution and on surfaces. The dye's strong emission in the orange-red region helps maintain visible contrast in fluorescence imaging experiments, including studies where background from unlabeled components is a concern.

2. Fluorescence Microscopy Imaging

BODIPY 576/589 supports fluorescence microscopy applications where an orange-red emitting dye is advantageous for multicolor imaging panels and for tracking labeled targets in fixed-cell or in vitro sample formats. Researchers use BODIPY 576/589-labeled conjugates to visualize labeled biomolecular assemblies, surface-associated structures, or tagged components within complex samples. Its BODIPY core is frequently selected when experiments require consistent fluorescence readout across imaging sessions, such as comparative imaging of labeled samples under matched acquisition settings.

3. Flow Cytometry Fluorescent Tagging

BODIPY 576/589 is frequently incorporated into fluorescent tagging strategies for flow cytometry workflows that rely on orange-red emission detection. In these applications, researchers prepare dye-labeled biomolecule conjugates or labeled particles to quantify fluorescence intensity shifts associated with binding, uptake, or surface association in cell-free or cell-based experimental systems. The dye's emission characteristics align well with common cytometer filter sets used for orange-red fluorophores, enabling multiplex designs where spectral separation from other channels is required.

4. Polymer And Biomaterial Labeling

BODIPY 576/589 is used to label polymers, hydrogels, and biomaterial surfaces to enable fluorescence-based tracking of material distribution, coating uniformity, and component mixing. Materials scientists incorporate the dye into polymer formulations or surface-functionalized constructs to visualize where labeled phases localize during fabrication, processing, or incubation experiments. This approach is also used to generate fluorescent materials for microscopy readouts and for quality-control style imaging of labeled biomaterial architectures.