BODIPY 493/503 carboxylic acid | CAS 216961-95-4

BODIPY 493/503 carboxylic acid is a BODIPY-family fluorescent dye bearing a carboxylic acid handle for conjugation to biomolecules and materials. Its emission in the green spectral region makes it a practical fluorophore for fluorescence labeling workflows where bright, compact dye scaffolds are advantageous. The carboxyl group enables coupling strategies that generate fluorescent conjugates for imaging and quantitative assays.

1. Protein And Peptide Labeling

BODIPY 493/503 carboxylic acid is frequently used to prepare fluorescently labeled proteins and peptides for fluorescence microscopy and fluorescence-based bioassays. Researchers typically couple the dye to lysine-containing biomolecules or to engineered biomolecular scaffolds to generate tracer conjugates that report on biomolecule distribution, binding to assay surfaces, or incorporation into multicomponent systems. The BODIPY core's high fluorescence efficiency and photophysical stability are commonly leveraged when labeling reagents must withstand routine washing and imaging workflows.

2. Fluorescent Conjugate Standards

BODIPY 493/503 carboxylic acid is also used as a reference fluorophore for assay development and instrument validation, including calibration of fluorescence readouts in plate readers and imaging systems. By preparing dye-conjugated materials or controlled dye solutions, laboratories can establish consistent fluorescence baselines for method optimization, normalization of signal across experimental runs, and evaluation of labeling efficiency in conjugation workflows. The carboxylic acid functionality supports preparation of defined conjugates that behave as stable fluorescent standards in experimental pipelines.

3. Biomaterial And Surface Functionalization

BODIPY 493/503 carboxylic acid supports fluorescence labeling of polymers, hydrogels, and other biomaterial platforms through covalent attachment via the carboxyl group. Materials researchers use the resulting fluorescently tagged surfaces to visualize coating uniformity, track material localization, and follow transport or adhesion behaviors in microscopy-based studies. This dye is particularly useful when a small, robust fluorophore is needed to create stable fluorescent materials compatible with common fluorescence imaging setups.

4. Fluorescence Imaging Tracing

BODIPY 493/503 carboxylic acid is applied as a green-emitting tracer for cellular and molecular imaging experiments where fluorescent labeling enables visualization of labeled species in complex biological samples. After conjugation to relevant carriers such as targeting ligands, affinity reagents, or macromolecular scaffolds, the dye helps researchers track localization patterns using fluorescence microscopy and related imaging modalities. The carboxyl functionality provides a convenient route to incorporate the fluorophore into diverse imaging reagents used for localization and distribution studies.