BDP FL-PEG4-TCO | CAS 2183473-16-5

BDP FL-PEG4-TCO is a strained-alkene (trans-cyclooctene, TCO) click handle bearing a PEG4 spacer that is used to build bioorthogonal conjugates for fluorescence-based molecular imaging and labeling workflows. In practice, researchers incorporate this reagent into fluorescent probe constructs where rapid TCO-tetrazine ligation enables modular attachment of fluorophores to biomolecules, targeting ligands, or imaging scaffolds under mild conditions compatible with many labeling pipelines.

1. Fluorescent Bioconjugation

BDP FL-PEG4-TCO is used by chemical biology and imaging groups to generate fluorescently labeled conjugates through TCO-tetrazine click chemistry, enabling efficient assembly of probe-biomolecule constructs. The PEG4 spacer helps reduce steric constraints during conjugation, supporting consistent fluorophore presentation on proteins, peptides, or other targeting components used in microscopy, binding studies, and imaging reagent development.

2. Targeted Imaging Probe Assembly

BDP FL-PEG4-TCO supports modular construction of targeted fluorescence imaging reagents where a TCO-functional fluorescent component is ligated to a tetrazine-bearing targeting moiety or carrier. Teams developing molecular imaging tools use this approach to rapidly swap targeting ligands while keeping the BDP fluorophore constant, facilitating iterative probe optimization for cellular imaging experiments and fluorescence readouts.

3. Surface and Material Functionalization

BDP FL-PEG4-TCO is applied in biomaterials and surface chemistry workflows to introduce a fluorescent click handle onto PEGylated or polymeric surfaces that will later be decorated via tetrazine ligation. Researchers use the TCO functionality to create fluorescence-enabled material labeling and tracking strategies, including preparation of fluorescently tagged hydrogels, coatings, and engineered interfaces for microscopy-based characterization and materials studies.

4. Fluorescence Labeling Standards

BDP FL-PEG4-TCO is also used as a practical fluorescent labeling component for assay and workflow development where a defined TCO-bearing fluorophore is needed for consistent conjugation chemistry. In laboratories optimizing tetrazine-click assembly steps, the reagent serves as a controllable fluorescent partner to evaluate labeling performance across different biomolecule formats and experimental conditions.