
BDP 630/650 lipid stain
| Catalog Number | F01-0265 |
| Category | BODIPY |
| Molecular Formula | C25H21BF2N2O3S |
| Molecular Weight | 478.32 |
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Product Introduction
BDP 630/650 lipid stain is a derivative of BDP 630/650 ethyl ester and can be used to stain lipids and other lipophilic compounds.
Chemical Information
Product Specification
Application
Chemical Information
| IUPAC Name | ethyl 2-[4-[(E)-2-(2,2-difluoro-12-thiophen-2-yl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)ethenyl]phenoxy]acetate |
| SMILES | [B-]1(N2C(=CC=C2C=CC3=CC=C(C=C3)OCC(=O)OCC)C=C4[N+]1=C(C=C4)C5=CC=CS5)(F)F |
| InChI | InChI=1S/C25H21BF2N2O3S/c1-2-32-25(31)17-33-22-12-6-18(7-13-22)5-8-19-9-10-20-16-21-11-14-23(24-4-3-15-34-24)30(21)26(27,28)29(19)20/h3-16H,2,17H2,1H3/b8-5+ |
| InChIKey | XAYPLEUJSQBEGK-VMPITWQZSA-N |
| Solubility | Soluble in DMSO, dichloromethane |
| Appearance | Deep Blue Glassy Solid |
Product Specification
| Excitation | 628 |
| Emission | 642 |
Application
BDP 630/650 lipid stain is a far-red fluorescent lipid labeling reagent built for imaging lipid-rich domains in biological samples. Its BODIPY-based fluorescence in the 630-650 nm region supports low-background visualization when paired with standard far-red fluorescence microscopy and fluorescence imaging workflows. The dye's lipophilic character enables membrane-associated staining, making it a common choice for researchers studying lipid distribution, membrane remodeling, and lipid droplet-adjacent structures.
1. Lipid Droplet Imaging
BDP 630/650 lipid stain is frequently used in fluorescence microscopy to visualize lipid storage structures and lipid-rich compartments in cultured cells. Researchers apply it to monitor changes in lipid organization during experimental treatments, including studies of lipid metabolism and membrane remodeling. The far-red emission helps reduce spectral overlap with common green and orange fluorophores, supporting multicolor imaging panels in cell biology workflows.
2. Membrane Lipid Localization
BDP 630/650 lipid stain supports membrane-associated staining for experiments where researchers need to map lipid distribution across cellular membranes. In microscopy-based studies, the reagent is used to compare relative lipid localization patterns between experimental conditions and to track redistribution during time-course imaging. Its 630-650 nm fluorescence output is particularly useful when imaging with instrumentation configured for far-red detection channels.
3. Multicolor Fluorescence Panels
BDP 630/650 lipid stain is commonly incorporated into multiplex fluorescence imaging strategies, where far-red spectral separation improves channel independence. Imaging teams use it alongside other organelle dyes or protein labels to correlate lipid-rich regions with cellular structures and biomolecular localization. This makes the reagent a practical staining component for microscopy workflows that require robust far-red readout and compatibility with standard fluorescence filter sets.
4. Fixed-Cell and Imaging Assays
BDP 630/650 lipid stain is used in fixed-sample imaging workflows where researchers want consistent lipid staining for quantitative microscopy. Cell imaging groups often employ it in assay development and phenotypic screening experiments that rely on fluorescence intensity readouts from lipid-rich compartments. The reagent's strong far-red emission supports downstream image analysis pipelines, including segmentation and intensity-based quantification in high-content microscopy environments.
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