BDP 630/650 carboxylic acid | CAS 2183512-02-7

BDP 630/650 carboxylic acid is a BODIPY-based fluorescent dye supplied with a carboxylic acid handle for downstream bioconjugation, enabling stable labeling of biomolecules and materials. Its red/orange spectral window (commonly used around the 630-650 nm region) makes it a practical fluorophore for multicolor fluorescence workflows where longer-wavelength channels help reduce spectral overlap. In conjugate formats, the dye is typically incorporated via amide/ester-forming coupling strategies to generate imaging-ready probes for microscopy and fluorescence-based assays.

1. Protein And Peptide Labeling

BDP 630/650 carboxylic acid is frequently used to prepare fluorescently labeled proteins and peptides for fluorescence imaging and quantitative fluorescence readouts. Researchers conjugate the carboxylic acid functionality to lysine- or amine-containing biomolecules to generate BODIPY-tagged reagents for tracking binding interactions, monitoring biomolecular localization, and benchmarking labeling performance in assay development. The dye's longer-wavelength emission supports multiplex experiments alongside green/yellow fluorophores, which is valuable for colocalization studies and panel-based imaging.

2. Fluorescence Microscopy Tracing

BDP 630/650 carboxylic acid is applied as a labeling component for fluorescence microscopy workflows that require a bright, photostable red-emitting dye for cellular and biomaterial imaging. In practice, conjugates prepared from the dye are used to visualize labeled targets such as surface-associated biomolecules on engineered materials, fluorescent tracers in microscopy studies, and multicolor imaging experiments where the 630-650 nm channel is advantageous. Imaging teams often rely on this dye to generate consistent staining reagents for fixed-sample workflows and imaging method development.

3. Flow Cytometry Dye Conjugates

BDP 630/650 carboxylic acid is used in the development of flow cytometry-compatible fluorescent conjugates, particularly when red-channel detection is preferred for multicolor panels. Conjugation of the carboxylic acid handle to targeting proteins or other amine-bearing reagents enables researchers to generate BODIPY-labeled probes for cell-surface or biomolecule-associated staining experiments. The dye's emission in the red region supports gating strategies that separate the signal from shorter-wavelength fluorophores, helping streamline multicolor cytometry assay design.

4. BODIPY-Based FRET Donor Labeling

BDP 630/650 carboxylic acid is also used as a fluorescent donor component in FRET-oriented reagent design, where BODIPY emission in the red/orange region can provide spectral overlap with complementary acceptors. In these workflows, researchers prepare donor-labeled biomolecular constructs or labeled assay reagents by coupling the dye's carboxylic acid functionality to the appropriate scaffold. The resulting donor conjugates are then incorporated into FRET assay development for proximity-dependent fluorescence readouts and molecular interaction studies, including format optimization for plate-based fluorescence measurements.