BDP 630/650 amine | CAS 2183473-00-7

BDP 630/650 amine is a BODIPY-based fluorescent dye supplied with an amine functional group for straightforward incorporation into fluorescent labeling workflows. Its red-orange emission profile makes it a practical choice for multicolor microscopy and fluorescence readouts where spectral separation from green channels is beneficial. Researchers typically use the amine handle to prepare dye conjugates for labeling biomolecules, building fluorescent probes, or functionalizing materials for imaging and quantification experiments.

1. Protein And Peptide Labeling

BDP 630/650 amine is used by chemical biology and proteomics groups to generate fluorescent protein and peptide conjugates for imaging and assay development. The dye's amine functionality enables coupling strategies that attach the fluorophore to lysine-containing proteins, peptides, or amine-terminated linkers, supporting downstream fluorescence microscopy and plate-based fluorescence measurements. This labeling format is frequently selected when a red-emitting BODIPY fluorophore is desired for tracking biomolecule distribution, monitoring binding interactions, or creating labeled standards for fluorescence assays.

2. Fluorescent Probe Construction

BDP 630/650 amine is incorporated into fluorescent probe designs where a stable, red-emitting BODIPY fluorophore is required as a reporter unit. In probe construction workflows, the amine handle is used to introduce the dye into larger sensing or targeting constructs, including conjugates that carry recognition elements such as peptides, affinity ligands, or reactive scaffolds. Fluorescent probe developers use this reagent to create imaging reagents for cellular or biochemical studies in which the dye serves as the optical readout component of a larger functional system.

3. Biomaterial And Surface Functionalization

BDP 630/650 amine is applied in biomaterials research to introduce red fluorescence into polymeric matrices, coatings, and surface-modified platforms. Materials scientists commonly use the amine functionality to tether the dye to amine-reactive surfaces or to dye-functionalize amine-terminated polymers and hydrogels, enabling visualization of material localization, coating coverage, or diffusion/retention behavior in fluorescence microscopy experiments. This approach is also used to prepare fluorescently labeled scaffolds and reagents for studying biomaterial interactions with cells or biomolecules under controlled laboratory conditions.

4. Multicolor Imaging References

BDP 630/650 amine is frequently used as a reference fluorophore component in multicolor fluorescence imaging experiments where a 630/650 nm-emitting dye improves channel separation. Imaging teams incorporate the dye into labeled conjugates or standards to facilitate spectral assignment, instrument setup, and experimental normalization across microscopy or fluorescence plate readers. In workflows that compare multiple fluorophores, BDP 630/650 amine provides a convenient red-channel reporter for consistent visualization and quantification alongside other spectrally distinct dyes.