BDP 581/591 amine | CAS 2183472-97-9

BDP 581/591 amine is a fluorescent labeling dye designed for covalent attachment through its amine-reactive functionality, enabling bright red/orange emission suitable for multicolor fluorescence workflows. In typical research settings, it is used to prepare fluorescent conjugates for imaging and quantitative assays where stable dye-biomolecule linkage is required, including labeling of proteins, peptides, and other primary-amine-containing targets. The dye's spectral position in the 580-590 nm region supports common fluorescence microscope and plate-reader configurations that collect red/orange channels.

1. Protein And Peptide Labeling

BDP 581/591 amine is frequently used by chemical biology and bioconjugation researchers to generate fluorescent protein and peptide conjugates for localization and binding studies. After conjugate preparation, the dye-labeled biomolecules are used in fluorescence microscopy to visualize distribution in fixed samples, in fluorescence-based binding assays, and in workflow development for fluorescence-tagged affinity reagents. Researchers also rely on the amine handle to support straightforward coupling to biomolecules bearing accessible nucleophilic groups, enabling consistent labeling for downstream imaging and quantification.

2. Fluorescence Microscopy Tracing

BDP 581/591 amine is applied in cellular and biomaterials imaging workflows where red/orange channel readout is advantageous for separating signals from green fluorophores. Labeled proteins, peptides, or other conjugates prepared with this dye are used as molecular tracers to follow adsorption, transport, or association with surfaces and matrices in microscopy experiments. In practice, the dye is selected for experiments requiring a robust fluorescent tag that can be imaged using standard filter sets aligned with its 581/591 emission window, supporting multichannel cellular imaging and co-localization studies.

3. Flow Cytometry Dye Conjugates

BDP 581/591 amine is used to construct fluorescent conjugates for flow cytometry panels that include a red/orange detection channel. Researchers prepare dye-labeled biomolecules as staining reagents for labeling targets on cells or for reporting binding events in suspension assays, then analyze stained samples on cytometers equipped to detect the dye's emission range. This application is particularly common in assay and reagent development settings where consistent covalent labeling helps reduce variability between batches of fluorescent staining reagents used for quantitative cytometry readouts.

4. Fluorescence-Based Bioassay Development

BDP 581/591 amine supports fluorescence assay development by providing a dye label for constructing fluorescent readouts in plate-based formats. Conjugates prepared using this dye are used as signal reporters in binding assays, substrate/interaction monitoring experiments, and other fluorescence-based screening workflows that require a stable fluorescent tag. The dye's emission in the 580-590 nm region makes it a practical choice for assay designs that use red/orange detection to minimize spectral overlap with common assay fluorophores, enabling multiplex-compatible assay layouts.