BDP 564/570 carboxylic acid | CAS 150173-89-0

BDP 564/570 carboxylic acid is a BODIPY-based fluorescent dye supplied as a carboxylic acid for conjugation-ready labeling workflows. Its bright visible emission in the orange-red region makes it well suited for fluorescence imaging and quantitative fluorescence assays where stable dye performance and covalent attachment to biomolecules are required. The carboxyl group enables straightforward incorporation into labeled probes and conjugates used across microscopy, flow cytometry, and fluorescence-based detection formats.

1. Protein Conjugation Labeling

BDP 564/570 carboxylic acid is frequently used by chemical biology and protein engineering groups to generate fluorescently labeled proteins for localization and binding studies. Researchers typically incorporate the dye into protein conjugates to track expression, uptake, or interaction partners in fluorescence microscopy and plate-based fluorescence assays. The carboxylic acid handle supports covalent dye attachment strategies that help reduce dye loss during washing steps common in imaging workflows.

2. Fluorescence Microscopy Imaging

BDP 564/570 carboxylic acid is applied in fluorescence microscopy for cellular and biomolecular imaging experiments that benefit from orange-red channel readouts. Imaging labs use dye-conjugated targeting reagents or labeled biomolecules to visualize distributions at the single-cell level, including colocalization experiments where the emission window is chosen to minimize overlap with other fluorophores. The dye's conjugation-ready functionality supports consistent staining of fixed or prepared samples used in microscopy pipelines.

3. Flow Cytometry Fluorescent Labeling

BDP 564/570 carboxylic acid supports flow cytometry workflows that rely on covalent fluorescent labeling for robust signal during sample processing. Flow cytometry users commonly prepare dye-labeled biomolecules for quantifying binding events, tracking biomaterial-associated components, or monitoring labeled populations in multiplex staining panels. The carboxylic acid form is leveraged to build stable conjugates that remain associated with the labeled entity through centrifugation and wash steps typical of cytometry sample preparation.

4. Fluorescent Assay Reagent Development

BDP 564/570 carboxylic acid is used by assay developers to construct fluorescence reagents for quantitative readouts in microplate formats. In fluorescence assay development, the dye is incorporated into labeled reporters such as binding reagents, detection conjugates, or fluorescent standards that are compatible with routine fluorescence instrumentation. The orange-red emission supports assay design choices where spectral separation from other assay components is important for reliable measurement of signal changes associated with binding or labeling events.