azido-Bodipy-FL-510 | CAS 1048369-35-2

azido-Bodipy-FL-510 is an azide-functionalized BODIPY fluorophore designed for click chemistry-enabled fluorescent labeling. Its bright visible emission around the green-to-orange boundary (commonly used with 510 nm-class detection) makes it well suited for fluorescence imaging and quantitative fluorescence workflows, while the azide handle enables efficient conjugation to alkyne-bearing biomolecules, linkers, and materials. Researchers use this reagent to build fluorescent conjugates and probes with defined attachment points for microscopy, flow cytometry, and assay development.

1. Biomolecule Labeling

azido-Bodipy-FL-510 supports site-defined fluorescent tagging of proteins, peptides, and other biomolecules through azide-alkyne click conjugation. In chemical biology and proteomics tool development, it is frequently incorporated into labeling strategies where a fluorophore is attached to an alkyne-functional biomolecule or to an alkyne-containing affinity reagent, enabling downstream fluorescence readout for localization studies, binding experiments, and conjugate tracking. The BODIPY core provides a compact, photostable fluorescent platform that is convenient for constructing fluorescent bioconjugates used in imaging and quantitative assays.

2. Fluorescence Microscopy

azido-Bodipy-FL-510 is used to generate fluorescent labeling reagents for fluorescence microscopy, including experiments that require robust, covalent attachment of a dye to a target scaffold. Typical workflows include preparing fluorescently labeled antibodies or affinity ligands (via alkyne-functional intermediates) for cellular or biochemical imaging, as well as labeling polymers, nanoparticles, or biomaterial surfaces to visualize distribution and association in microscopy-based studies. The azide functionality allows researchers to integrate the fluorophore into multi-component staining schemes where click-compatible partners are already present, helping maintain consistent labeling chemistry across experiments.

3. Flow Cytometry Staining

azido-Bodipy-FL-510 is commonly used to prepare fluorescent conjugates for flow cytometry panels where a BODIPY dye in the 510 nm-class range provides a convenient channel for quantitative measurements. By clicking the azide dye onto alkyne-bearing targeting reagents or labeling handles, users can generate covalently attached fluorescent probes for cell-surface or biomolecule staining workflows. This approach is particularly useful when consistent conjugation chemistry is required across batches of reagents, such as in assay development and screening-oriented experiments that rely on reproducible fluorescence intensity distributions.

4. Fluorescent Probe Construction

azido-Bodipy-FL-510 is well suited for constructing fluorescence probes and labeled reporters that incorporate an azide-bearing fluorophore into custom probe architectures. Researchers use it to build modular fluorescent constructs by coupling the azide handle to alkyne-functional linkers, targeting motifs, or sensor scaffolds, enabling rapid generation of probe variants for method development. In fluorescence assay development, the dye's integration via click-compatible chemistry helps ensure that the fluorophore is positioned within a defined conjugate framework used for monitoring binding, labeling kinetics, or reporter signal generation in plate-based and imaging assays.